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Product Info Summary
| SKU: | A07814 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-THEM4 Antibody
SKU/Catalog Number
A07814
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-THEM4 Antibody (Catalog # A07814). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
THEM4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-THEM4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07814)
Host
Rabbit
Contents
THEM4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
THEM4 antibody was raised against an 18 amino acid synthetic peptide near the center of human THEM4. The immunogen is located within amino acids 60 - 110 of THEM4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.
Reactive Species
A07814 is reactive to Them4 in Human, Mouse, Rat
Applications
A07814 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
26.031kDa
Background of Them4
THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
THEM4 antibody can be used for detection of THEM4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of THEM4 in human liver tissue lysate with THEM4 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of THEM4 in human liver tissue with THEM4 antibody at 2.5 μg/mL.
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Immunofluorescence of THEM4 in human liver tissue with THEM4 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Them4 (Source: Uniprot.org, NCBI)
Gene Name
Them4
Full Name
Acyl-coenzyme A thioesterase THEM4
Weight
26.031kDa
Superfamily
THEM4/THEM5 thioesterase family
Alternative Names
C-terminal modulator protein; CTMPCarboxyl-terminal modulator protein; MGC29636; thioesterase superfamily member 4 Them4|2700077M13Rik, 4921507I02Rik|thioesterase superfamily member 4|acyl-coenzyme A thioesterase THEM4|acyl-CoA thioesterase THEM4|carboxyl-terminal modulator protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Them4, check out the Them4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Them4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-THEM4 Antibody (A07814)
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No publications found for A07814
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