Anti-Thrombospondin 2 Antibody

Product Name

Anti-Thrombospondin 2 Antibody

View all Thrombospondin-2 Antibodies

SKU/Catalog Number

A03253

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Thrombospondin 2 Antibody catalog # A03253. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Thrombospondin 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03253)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

1B1C4

Isotype

IgG

Immunogen

The antiserum was produced against synthesized peptide derived from the C-terminal region of human THBS2 at amino acid range 1000-1050

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03253 is reactive to THBS2 in Human, Mouse, Rat

Applications

A03253 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

129.991kDa

Background of Thrombospondin-2

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 7 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 7 seems to be involved proteosomal degradation of proteins involved in endothelial proliferation and/or differentiation. Cullin 7 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Cullin 7 interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000

Validation Images & Assay Conditions

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Western blot analysis of mouse-heart lysis using THBS2 antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

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Immunohistochemical analysis of paraffin-embedded rat-testis, antibody was diluted at 1:100

Gene/Protein Information For THBS2 (Source: Uniprot.org, NCBI)

Alternative Names

THBS2; thrombospondin 2; Thrombospondin2; Thrombospondin-2; TSP-2; TSP2thrombospondin-2 THBS2 TSP2 thrombospondin 2 thrombospondin-2

For more info on THBS2, check out the THBS2 Infographic

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In this infographic, you will see the following information for THBS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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