Anti-Thymidylate Synthase (5-FU Resistance Marker) TYMS Monoclonal Antibody

Product Name

Anti-Thymidylate Synthase (5-FU Resistance Marker) TYMS Monoclonal Antibody

View all Thymidylate Synthase Antibodies

SKU/Catalog Number

M04320

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Thymidylate Synthase (5-FU Resistance Marker) TYMS Monoclonal Antibody (Catalog # M04320). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Thymidylate Synthase (5-FU Resistance Marker) TYMS Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04320)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: TS106 + TMS715

Isotype

IgG1, kappa + IgG1, kappa

Immunogen

Recombinant human thymidylate synthase (TS106 & TMS715)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M04320 is reactive to TYMS in Human

Applications

M04320 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

35.716kDa

Background of Thymidylate Synthase

This monoclonal antibody recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Anti-Thymidylate Synthase Monoclonal Antibody (TS106 + TMS715).

Gene/Protein Information For TYMS (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.1.1.45; HsT422; MGC88736; thymidylate synthase; thymidylate synthetase; TMSTsase; TSase; TSHST422 TYMS HST422, TMS, TS thymidylate synthetase thymidylate synthase|TSase

For more info on TYMS, check out the TYMS Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TYMS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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