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TIGAR antibody (A05811-3) performs well in ELISA, IF, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of TIGAR using anti-TIGAR antibody (A05811-3).
TIGAR was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TIGAR Antibody (A05811-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
TIGAR was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TIGAR Antibody (A05811-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-TIGAR Antibody Picoband®
TIGAR antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, IF, IHC, WB |
Tested Applications
TIGAR antibody was positively detected by WB in:
rat brain tissue, rat skeletal muscle tissue, rat heart tissue, rat H9C2 whole cell, mouse brain tissue, mouse skeletal muscle tissue, mouse heart tissue, mouse C2C12 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TIGAR antibody was positively detected by IHC in:
rat brain tissue, mouse cerebellum tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TIGAR antibody was positively detected by IF in:
mouse cerebellum tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse, Rat<
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse, Rat
Immunofluorescence
5 μg/ml
Mouse
ELISA
0.1-0.5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Tested
Recommend
Product Information
Product Name
Anti-TIGAR Antibody Picoband®
View all TIGAR Antibodies
View all TIGAR Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived mouse TIGAR recombinant protein (Position: M1-H269). Mouse TIGAR shares 72.8% amino acid (aa) sequence identity with human TIGAR.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
30 kDa
Calculated Molecular Weight
29.2 kDa
Background of TIGAR
Enables fructose-2,6-bisphosphate 2-phosphatase activity. Involved in positive regulation of pentose-phosphate shunt and response to ischemia. Acts upstream of or within several processes, including fructose 2,6-bisphosphate metabolic process; negative regulation of catabolic process; and positive regulation of cardiac muscle cell apoptotic process. Located in mitochondrion. Is expressed in several structures, including brain; heart; immune system; and liver. Orthologous to human TIGAR (TP53 induced glycolysis regulatory phosphatase).
Cite This Product
Anti-TIGAR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05811-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
90%
High Homology
Rabbit
73%
Moderate Homology
Human
72%
Moderate Homology
Monkey
72%
Moderate Homology
Dog
71%
Moderate Homology
Cow
71%
Moderate Homology
Sheep
66%
Low homology
Zebrafish
41%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TIGAR antibody
Immunohistochemistry (IHC) protocol for TIGAR antibody
Immunofluorescence (IF) protocol for TIGAR antibody
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Product Promise
We promise Anti-TIGAR Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A05811-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026