Anti-TIGIT Monoclonal Antibody [4A10]

Product Name

Anti-TIGIT Monoclonal Antibody [4A10]

View all TIGIT Antibodies

SKU/Catalog Number

M01962-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TIGIT Monoclonal Antibody [4A10] (Catalog # M01962-1). Tested in ELISA, WB, IHC-P, ICC, IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

TIGIT antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TIGIT Monoclonal Antibody [4A10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01962-1)

Host

Mouse

Contents

TIGIT Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Clonality

Monoclonal

Clone Number

Clone: 4A10

Isotype

IgG1

Immunogen

TIGIT antibody was raised against the extracellular domain of human TIGIT

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.

Reactive Species

M01962-1 is reactive to TIGIT in Human

Applications

M01962-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

26.319kDa

Background of TIGIT

The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TIGIT antibody can be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 1 μg/mL. For flow cytometry at 1 μg/ml. For immunocytochemistry at 1 μg/mL. For Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.

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Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 μg/ml.

Green: TIGIT Antibody [4A10] (RF16054)
Blue: DAPI staining

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Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 μg/ml.

Green: TIGIT Antibody [4A10] (RF16054)
Blue: DAPI staining

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Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 μg/ml.

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Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.

Gene/Protein Information For TIGIT (Source: Uniprot.org, NCBI)

Alternative Names

T cell immunoreceptor with Ig and ITIM domains; TIGIT; VSIG9; VSTM3; WUCAM TIGIT VSIG9, VSTM3, WUCAM T cell immunoreceptor with Ig and ITIM domains T-cell immunoreceptor with Ig and ITIM domains|V-set and immunoglobulin domain containing 9|V-set and immunoglobulin domain-containing protein 9|V-set and transmembrane domain containing 3|V-set and transmembrane domain-containing protein 3|VSIG9, VSTM3|Washington University cell adhesion molecule

For more info on TIGIT, check out the TIGIT Infographic

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In this infographic, you will see the following information for TIGIT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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