Product Info Summary
| SKU: | A05651 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-TIS11B ZFP36L1 Antibody
SKU/Catalog Number
A05651
Size
100μl
Form
Liquid
Description
Boster Bio Anti-TIS11B ZFP36L1 Antibody catalog # A05651. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TIS11B ZFP36L1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05651)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human TISB. AA range:58-107
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05651 is reactive to ZFP36L1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
36.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05651 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of various cells using TIS11B Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Click image to see more details
Western blot analysis of lysates from Jurkat, HeLa, A549, and LOVO cells, using TISB Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TISB Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TIS11B ZFP36L1 Antibody (A05651)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-TIS11B ZFP36L1 Antibody
Question
Would anti-TIS11B antibody A05651 work on canine WB with liver?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
Our lab technicians have not tested anti-TIS11B antibody A05651 on canine. You can run a BLAST between canine and the immunogen sequence of anti-TIS11B antibody A05651 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-01
Question
I see that the anti-TIS11B antibody A05651 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-09
Question
Our lab want to know about to test anti-TIS11B antibody A05651 on mouse ovary for research purposes, then I may be interested in using anti-TIS11B antibody A05651 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
The products we sell, including anti-TIS11B antibody A05651, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-06
Question
We are currently using anti-TIS11B antibody A05651 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
E. Johnson
Verified customer
Asked: 2019-09-04
Answer
The anti-TIS11B antibody (A05651) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-04
Question
I have a question about product A05651, anti-TIS11B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
V. Anderson
Verified customer
Asked: 2019-03-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05651 anti-TIS11B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-11
Question
Is this A05651 anti-TIS11B antibody reactive to the isotypes of ZFP36L1?
C. Williams
Verified customer
Asked: 2015-09-11
Answer
The immunogen of A05651 anti-TIS11B antibody is Synthesized peptide derived from human TIS11B around the non-phosphorylation site of S92. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-09-11
Question
I was wanting to use your anti-TIS11B antibody for WB for mouse ovary on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse ovary identification?
G. Huang
Verified customer
Asked: 2015-06-12
Answer
As indicated on the product datasheet, A05651 anti-TIS11B antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-06-12

