Click image to see more details
Product Info Summary
| SKU: | M02460 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TLE1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02460
BM5726 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TLE1 Rabbit Monoclonal Antibody catalog # M02460. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TLE1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02460)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFEA-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TLE 1 Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling.
Reactive Species
M02460 is reactive to TLE1 in Human, Mouse, Rat
Observed Molecular Weight
83 kDa
Calculated molecular weight
83.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02460 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: SH-SY5Y cell
Validation Images & Assay Conditions
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Western blot analysis of TLE 1 expression in SH-SY5Y cell lysate.
Specific Publications For Anti-TLE1 Rabbit Monoclonal Antibody (M02460)
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Customer Q&As
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7 Customer Q&As for Anti-TLE1 Rabbit Monoclonal Antibody
Question
Does anti-TLE1 Monoclonal antibody M02460 work on goat WB with colon kidney?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
Our lab technicians have not tested anti-TLE1 Monoclonal antibody M02460 on goat. You can run a BLAST between goat and the immunogen sequence of anti-TLE1 Monoclonal antibody M02460 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat colon kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-15
Question
What sample target on Western Blot application was tested with M02460?
Verified customer
Asked: 2020-01-03
Answer
Western blot analysis of TLE 1 expression in SH-SY5Y cell lysate.
Boster Scientific Support
Answered: 2020-01-16
Question
I was wanting to use your anti-TLE1 Monoclonal antibody for IF for mouse colon kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse colon kidney identification?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
As indicated on the product datasheet, M02460 anti-TLE1 Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse colon kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-17
Question
Is this M02460 anti-TLE1 Monoclonal antibody reactive to the isotypes of TLE1?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
The immunogen of M02460 anti-TLE1 Monoclonal antibody is A synthesized peptide derived from human TLE 1 Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon kidney using anti-TLE1 Monoclonal antibody M02460. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-02
Question
Do you have a BSA free version of anti-TLE1 Monoclonal antibody M02460 available?
J. Brown
Verified customer
Asked: 2018-02-09
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TLE1 Monoclonal antibody M02460 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-09
Question
We are currently using anti-TLE1 Monoclonal antibody M02460 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-26
Answer
The anti-TLE1 Monoclonal antibody (M02460) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-26
