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TLR4 antibody (PA1484) performs well in IHC, WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 74 Publications

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Anti-TLR4 antibody, PA1484, IHC(P)
IHC(P): Human Lung Cancer Tissue

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Anti-Toll-like receptor 4 TLR4 Antibody Picoband®

TLR4 antibody

74 Publications

Reactive Species	Host	Application
Human	Rabbit	IHC, WB

Tested Applications

TLR4 antibody was positively detected by WB in:

HELA Cell, SMMC Cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

TLR4 antibody was positively detected by IHC in:

Human Lung Cancer Tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Monkey

Predicted

Product Information

Product Name

Anti-Toll-like receptor 4 TLR4 Antibody Picoband®
View all TLR4 Antibodies

Form

Lyophilized

Uniprot

O00206

Gene ID

7099

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human TLR4.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

80 kDa

Calculated Molecular Weight

95.7 kDa

Background of TLR4

TLR4, Toll-like receptor 4, is a protein that in humans is encoded by the TLR4 gene. TLR 4 is a toll-like receptor. TLR4, the human homolog of Drosophila Toll, is a type I transmembrane protein with an extracellular domain consisting of a leucine-rich repeat region and an intracellular domain homologous to that of human interleukin-1 receptor. The TLR4 gene is mapped to chromosome 9q32-q33 by fluorescence in situ hybridization.It detects lipopolysaccharide from Gram-negative bacteria and is thus important in the activation of the innate immune system.The protein encoded by this gene is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.

Cite This Product

Anti-Toll-like receptor 4 TLR4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1484)

Protocols

Western Blot protocol for TLR4 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for TLR4 antibody

Review Protocol

Publications

Peripheral blood exosomal Hsa-miR-335-3p alleviates burn-induced acute lung injury by targeting TLR4 and the PI3K/AKT/NF-κB pathway

Published: 2026-08-26

Journal: IMMUNOBIOLOGY

Application: WB

Impact Factor: 3.10

Species: Human

PMID: 42673791

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Anti-Toll-like receptor 4 TLR4 Antibody Picoband®

TLR4 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Peripheral blood exosomal Hsa-miR-335-3p alleviates burn-induced acute lung injury by targeting TLR4 and the PI3K/AKT/NF-κB pathway

Signature of immune infiltration‑related ferroptosis genes to predict the prognosis of patients with osteosarcoma

Tanshinone IIA and hydroxy safflower yellow A reduce cerebral injury via TLR4/NF-κB pathway in rats

MiR-24 regulates obstructive pulmonary disease in rats via S100A8

Corilagin relieves atherosclerosis via the toll-like receptor 4 signaling pathway in vascular smooth muscle cells

Network pharmacology combined with molecular docking and experimental validation to explore the potential mechanism of Cinnamomi ramulus against ankylosing spondylitis

Total glycosides of Rhodiola rosea L. attenuate LPS-induced acute lung injury by inhibiting TLR4/NF-κB pathway

Therapeutic Effect of C-C Chemokine Receptor Type 1 (CCR1) Antagonist BX471 on Allergic Rhinitis

A study of diabetes-induced erectile dysfunction treated with human umbilical cord mesenchymal stem cells

Protective Effects of Selenium Nanoparticle-Enriched Lactococcus lactis NZ9000 against Enterotoxigenic Escherichia coli K88-Induced Intestinal Barrier Damage in Mice | Applied and Environmental Microbiology

Exploring the Mechanism of Resveratrol in Reducing the Soft Tissue Damage of Osteoarthritis Based on Network Pharmacology and Experimental Pharmacology

Serum‑derived exosomes from house dust mite‑sensitized guinea pigs contribute to inflammation in BEAS‑2B cells via the TLR4‑NF‑κB pathway

Danshensu alleviates pseudo-typed SARS-CoV-2 induced mouse acute lung inflammation

N-acetylserotonin alleviated the expression of interleukin-1β in retinal ischemia–reperfusion rats via the TLR4/NF-κB/NLRP3 pathway

Melatonin alleviates Ochratoxin A-induced liver inflammation involved intestinal microbiota homeostasis and microbiota-independent manner

HMGB1 is a key factor for tamoxifen resistance and has the potential to predict the efficacy of CDK4/6 inhibitors in breast cancer

Enhanced cytokine expression and upregulation of inflammatory signaling pathways in broiler chickens affected by wooden breast myopathy

Tetramethylpyrazine attenuates endotoxin-induced retinal inflammation by inhibiting microglial activation via the TLR4/NF-κB signalling pathway

HMGB1 promoted P-glycoprotein at the blood-brain barrier in MCAO rats via TLR4/NF-κB signaling pathway

Autophagy inhibits TLR4-mediated invasiveness of oral cancer cells via the NF-κB pathway

Cryptochlorogenic acid attenuates LPS-induced inflammatory response and oxidative stress via upregulation of the Nrf2/HO-1 signaling pathway in RAW 264.7 macrophages

Anti-influenza A virus mechanism of three representative compounds from Flos Trollii via TLRs signaling pathways

Physicochemical characterization of polysaccharide from the leaf of Dendrobium officinale and effect on LPS induced damage in GES-1 cell

Effect of seasonal variance on intestinal epithelial barriers and the associated innate immune response of the small intestine of the Chinese soft-shelled turtles

Ochratoxin A induces liver inflammation: involvement of intestinal microbiota

H19 promote calcium oxalate nephrocalcinosis-induced renal tubular epithelial cell injury via a ceRNA pathway

Effects of Polygonatum sibiricum Polysaccharides (PSP) on Human Esophageal Squamous Cell Carcinoma (ESCC) via NF-κB Signaling Pathway

Effects of the combination of tanshinone IIA and puerarin on cardiac function and inflammatory response in myocardial ischemia mice

A Study of the Immunoregulatory Function of TLR3 and TLR4 on Mesenchymal Stem Cells in Ankylosing Spondylitis

Effect of curcumin supplementation on TLR4 mediated non-specific immune responses in liver of laying hens under high-temperature conditions

Red yeast rice ameliorates high-fat diet-induced atherosclerosis in Apoe−/− mice in association with improved inflammation and altered gut microbiota composition

Bone marrow stromal cells improved functional recovery in spinal cord injury rats partly via the Toll‑like receptor‑4/nuclear factor‑κB signaling pathway

Responses of Intestinal Mucosal Barrier Functions of Rats to Simulated Weightlessness

Effects of Periostracum Cicadae on Cytokines and Apoptosis Regulatory Proteins in an IgA Nephropathy Rat Model

Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling

Salvia przewalskii extract of total phenolic acids inhibit TLR4 signaling activation in podocyte injury induced by puromycin aminonucleoside in vitro

Expression of Toll‑like receptor 4 on mast cells in gingival tissues of human chronic periodontitis

Regulation of angiotensin II-induced B-cell lymphoma-2-associated athanogene 3 expression in vascular smooth muscle cells

Lipopolysaccharide enhances TGF-β1 signalling pathway and rat pancreatic fibrosis

Allicin Alleviates Reticuloendotheliosis Virus-Induced Immunosuppression via ERK/Mitogen-Activated Protein Kinase Pathway in Specific Pathogen-Free Chickens

The protective effects of magnolol on acute trinitrobenzene sulfonic acid‑induced colitis in rats

Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-κB signaling in an IL-6-dependent manner

TLR4 signaling is activated in ventilator-induced diaphragm dysfunction in rats

Regulation on Toll-like Receptor 4 and Cell Barrier Function by Rab26 siRNA-loaded DNA Nanovector in Pulmonary Microvascular Endothelial Cells

Orientin Attenuates Cerebral Ischemia/Reperfusion Injury in Rat Model through the AQP-4 and TLR4/NF-κB/TNF-α Signaling Pathway

Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury

Preventive and therapeutic effect of simvastatin on secondary inflammatory damage of rats with cerebral hemorrhage

Characterization of acetaminophen-induced cytotoxicity in target tissues

Differences in Proinflammatory Property of Six Subtypes of Peroxiredoxins and Anti-Inflammatory Effect of Ligustilide in Macrophages

Expression of TLR2/4 on Epididymal Spermatozoa of the Chinese Soft-Shelled Turtle Pelodiscus sinensis During the Hibernation Season

Up-regulated TLR2 and TLR4 expressions in liver and spleen during acute murine T. gondii infection

Prognostic value of toll-like receptor 4 and nuclear factor-κBp65 in oral squamous cell carcinoma patients

Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir

Bletilla striata polysaccharide inhibits angiotensin II-induced ROS and inflammation via NOX4 and TLR2 pathways

Knockdown of versican V1 induces a severe inflammatory response in LPS-induced acute lung injury via the TLR2-NF-κB signaling pathway in C57BL/6J mice

Toll-like receptor-2 and -4 are associated with hyperlipidemia

High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy

Expression of TLR2/4 in the sperm-storing oviduct of the Chinese soft-shelled turtle Pelodiscus sinensis during hibernation season

Protective effects of Radix Astragali injection on multiple organs of rats with obstructive jaundice

Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response

Upregulation of Toll‑like receptor 2 expression in colorectal cancer infected by human cytomegalovirus

Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor κB and Toll-Like Receptor 4 Expression in Rats with Acute Necrotizing Pancreatitis

Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis

The anti-inflammation effect of baicalin on experimental colitis through inhibiting TLR4/NF-κB pathway activation

TLR4 is constitutively expressed in chick thymic epithelial cells

Effect of TLR4 and B7-H1 on immune escape of urothelial bladder cancer and its clinical significance.

Salvianolic acid B inhibits the TLR4-NFκB-TNFα pathway and attenuates neonatal rat cardiomyocyte injury induced by lipopolysaccharide