Toll-like receptor 4 Antibodies

AND TLR4 ELISA kits, Toll-like receptor 4 Proteins

Many biological assays use antibodies to detect Toll-like receptor 4, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Toll-like receptor 4 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Toll-like receptor 4 antibodies...

We validate the specificity of these antibodies to Toll-like receptor 4 by testing them on tissues known to express TLR4 positively and negatively. Browse below to find the TLR4 antibody that suites your experiment. We have 7 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for Toll-like receptor 4? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the TLR4 Western Blot Guide

Designing an IHC experiment for Toll-like receptor 4? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the TLR4 IHC Guide

TLR4 Infographic by Boster Bio

All TLR4 Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-Toll-like receptor 4 TLR4 Antibody Picoband®

73 Citations

Anti-TLR4 antibody, PA1484, Western blottingLane 1: HELA Cell Lysate Lane 2: SMMC Cell Lysate

Human

IHC, WB

$370
Cat # PA1484
100 μg/vial

Anti-Tlr4 Antibody Picoband®

19 Citations

Western blot analysis of Tlr4 using anti-Tlr4 antibody (A00017-3)

Mouse

ELISA, WB

$370
Cat # A00017-3
100 μg/vial

Anti-TLR4 Antibody Picoband®

2 Citations

Western blot analysis of TLR4 using anti-TLR4 antibody (A00017-2)

Flow Cytometry analysis of U937 cells using anti-TLR4 antibody (A00017-2)

Human

ELISA, Flow Cytometry, WB

$370
Cat # A00017-2
100 μg/vial

Anti-Protein canopy homolog 3 CNPY3 Antibody

Western blot analysis of extracts of various cell lines, using CNPY3 antibody at 1:1000 dilution

Immunohistochemistry of paraffin-embedded mouse kidney using CNPY3 antibody at dilution of 1:100

Human, Mouse

IHC, WB

$405
Cat # A10971
100ul

Anti-TLR4 Reference Antibody(Paridiprubart)

The purity of Anti-TLR4 Reference Antibody (Paridiprubart)is more than 95%

Human

ELISA, Flow Cytometry, Functional Assay, Kinetics

$486
Cat # M00017
100 μg

TLR4 (NM_138554) Human Over-expression Lysate

Human

$960
Cat # LS007099
20 µg, 100 µg

TLR4/IL-8 Luciferase Reporter-HeLa Cell Line

Fig-1: Induction of TLR4 activity by LPS in TLR4/IL-8 HeLa cells

Functional Assay

$7280
Cat # RC1024
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

TLR4 Overview

Facts about Toll-like receptor 4.

TLR4 3D structure. Source: alphafold

Toll-like receptor 4 (TLR4)

Acts via MYD88, TIRAP and TRAF6, leading to NF- kappa-B activation, cytokine secretion and the inflammatory reaction (PubMed:9237759, PubMed:10835634, PubMed:27022195). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+).

Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species-specific (PubMed:20711192). Both M.

Gene Information

Human
Gene Name:	TLR4
Uniprot:	O00206
Entrez:	7099

Family

Belongs to:

Toll-like receptor family

Alternative Names

76B357.1; ARMD10; CD284 antigen; CD284; hTollhomolog of Drosophila toll; tlr4 76B357.1; TLR4 facs; TLR4 flow cytometry; TLR4 human; TLR4 ihc; TLR4 western blot; TLR4; TOLL; toll-like receptor 4

Molecular Weight

Mass (kDA):
95.68 kDA

Genomic Context


Human

Location:	9q33.1
Sequence:	9; NC_000009.12 (117704403..117724735)

Tissue Specificity

Highly expressed in placenta, spleen and peripheral blood leukocytes (PubMed:9435236, PubMed:9237759). Detected in monocytes, macrophages, dendritic cells and several types of T-cells (PubMed:9237759, PubMed:27022195).

Subcellular Localizations

Cell membrane; Single-pass type I membrane protein. Early endosome. Cell projection, ruffle. Upon complex formation with CD36 and TLR6, internalized through dynamin-dependent endocytosis (PubMed:20037584). Colocalizes with RFTN1 at cell membrane and then together with RFTN1 moves to endosomes, upon lipopolysaccharide stimulation.

Diseases

Inflammation
Innate Immune Response
Inflammatory Response
Infective Disorder
Systemic Infection
Malignant Neoplasms
Bacterial Infections
Pneumonia
Neoplasms
Atherosclerosis
Tissue Adhesions
Autoimmune Reaction
Ischemia

Colitis
Salmonella Infections
Reperfusion Injury
Septic Shock
Asthma
Arthritis
Obesity

Interacting Proteins

LY96
MYD88
NOX4
SIGLEC5
SIGLEC9

TICAM2
TIRAP
TLR6
TNC

Pathways

Immune Response
Secretion
Inflammatory Response
Cytokine Production
Pathogenesis
Innate Immune Response
Cell Activation
Phagocytosis
Cell Proliferation
Cell Death
Cytokine Secretion
Virulence
Macrophage Activation

Response To Lipopolysaccharide
Localization
Reverse Transcription
Adaptive Immune Response
Sensitization
Chemokine Production
Transport

PTMs

Phosphorylation
Cleavage
Methylation
Ubiquitination
Glycosylation
Acylation
Oxidation
Acetylation
Dephosphorylation
Biotinylation

Palmitoylation
Reduction
Myristoylation
Nitrosylation
Deglycosylation
Carboxylation
Farnesylation
Deacetylation
Phosphorothioation
Hydroxylation

Research Areas

Adaptive Immunity
Cytokine Research
Immunology
Innate Immunity
Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TLR4

References

PMID: 9237759 by Medzhitov R., et al. A human homologue of the Drosophila Toll protein signals activation of adaptive immunity.

PMID: 9435236 by Rock F.L., et al. A family of human receptors structurally related to Drosophila Toll.

30 Publications of Boster Toll-like receptor 4 Antibodies & ELISA Kits

PubMed ID: 10.1016/j.jtherbio.2019.07.003, Effect of curcumin supplementation on TLR4 mediated non-specific immune response...
PubMed ID: 34000276, Liu J,Zhang N,Zhang M,Yin H,Zhang X,Wang X,Wang X,Zhao Y.N-acetylserotonin allev...
PubMed ID: -, Daiyang Xia,Lin Yang,Yu Li,Jianying Chen,Xiufen Zhang,Heng Wang,Shuangshuang Zha...
PubMed ID: 28744333, Regulation on Toll-like Receptor 4 and Cell Barrier Function by Rab26 siRNA-load...
PubMed ID: 29804125, Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Co...
PubMed ID: 29442011, Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by i...
PubMed ID: 29312337, Allicin Alleviates Reticuloendotheliosis Virus-Induced Immunosuppression via ERK...
PubMed ID: 28424593, Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/R...
PubMed ID: 27275821, Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therap...
PubMed ID: 27109786, Knockdown of versicanV1 induces a severe inflammatory response in LPS-induced ac...
PubMed ID: 26645545, Toll-like receptor 4 contributes to chronic itch, alloknesis, and spinal astrocy...
PubMed ID: 29178911, Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 express...
PubMed ID: 26664692, Expression of TLR2/4 in the sperm?storing oviduct of the Chinese soft?shelled tu...
PubMed ID: 26468333, High glucose induces and activates Toll-like receptor 4 in endothelial cells of...
PubMed ID: 26053067, Increased Thymic Cell Turnover under Boron Stress May Bypass TLR3/4 Pathway in ...
PubMed ID: 29424488, Sun L, Xiu M, Wang S, Brigstock DR, Li H, Qu L, Gao R. J Cell Mol Med. 2018 Apr;...
PubMed ID: 24460643, Wang Y, Ge P, Yang L, Wu C, Zha H, Luo T, Zhu Y. J Neuroinflammation. 2014 Jan 2...
PubMed ID: 22683883, Tu Ct, Yao Qy, Xu Bl, Wang Jy, Zhou Ch, Zhang Sc. Food Chem Toxicol. 2012 Sep;50...
PubMed ID: 23656243, Xu N, Yu Zh, Yao Qs, Wang Zq, Qu Hl, Sun Y, Liu J, Jin Cz. J Immunoassay Immunoc...
PubMed ID: 22749378, Fu Hy, Li C, Yang W, Gai Xd, Jia T, Lei Ym, Li Y. Acta Histochem. 2013 Mar;115(2...
PubMed ID: 23411097, Wang Yz, Tian Ff, Liu H, Zhang W, Li J, Xiao B, Zhou Wb. J Neuroimmunol. 2013 Ap...

*Showing only the more recent 20. More publications can be found for each product on its corresponding product page