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Tlr4 antibody (A00017-3) performs well in ELISA, WB and shows reactivity with Mouse.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 20 Publications

a00017 3 tlr4 primary antibodies wb testing 1

Western blot analysis of Tlr4 using anti-Tlr4 antibody (A00017-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tlr4 antigen affinity purified polyclonal antibody (Catalog # A00017-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tlr4 at approximately 96 kDa. The expected band size for Tlr4 is at 96 kDa.

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Anti-Tlr4 Antibody Picoband®

Tlr4 antibody

20 Publications

Reactive Species	Host	Application
Mouse	Rabbit	ELISA, WB

Tested Applications

Tlr4 antibody was positively detected by WB in:

mouse RAW2647 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Reactive Data

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ELISA

Mouse

Tested

Recommend

Product Information

Product Name

Anti-Tlr4 Antibody Picoband®
View all TLR4 Antibodies

Form

Lyophilized

Uniprot

Q9QUK6

Gene ID

21898

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Tlr4 recombinant protein (Position: N26-E827).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Observed Molecular Weight

96 kDa

Calculated Molecular Weight

95.5 kDa

Background of Tlr4

Toll-like receptor 4 is a protein that in humans is encoded by the TLR4 gene. This gene belongs to the evolutionarily-conserved Toll-like receptor family, whose members are type-1 transmembrane proteins that are involved in innate immunity. Toll-like receptors are characterized by an extracellular leucine-rich repeat domain that functions in ligand recognition and an intracellular toll/interleukin-1 receptor-like domain that is crucial for signal transduction. The receptor encoded by this gene mediates the innate immune response to bacterial lipopolysaccharide, a major component of the outer membrane of Gram-negative bacteria, through synthesis of pro-inflammatory cytokines and chemokines. In addition, this protein can recognize other pathogens from Gram-negative and Gram-positive bacteria as well as viral components. Mice deficient in this gene display a number of immune response-related phenotypes including hyporesponsiveness to bacterial lipopolysaccharide and increased levels of respiratory syncytial virus compared to controls.

Cite This Product

Anti-Tlr4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00017-3)

Protocols

Western Blot protocol for Tlr4 antibody

Review Protocol

Publications

SEM1 Downregulates HSPA8 to Suppress TLR4/MyD88/NF-κB Signaling and Alleviate Myocardial I/R Injury

Published: 2026-07-27

Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Application: WB

Impact Factor: 5.60

Species: Rat

PMID: 42589368

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Anti-Tlr4 Antibody Picoband®

Tlr4 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

SEM1 Downregulates HSPA8 to Suppress TLR4/MyD88/NF-κB Signaling and Alleviate Myocardial I/R Injury

Anti-asthmatic effects and ingredient annotation of the active fraction from Ganoderma lucidum

Skin bacteriota ameliorates androgenetic alopecia via harmonizing skin immuno inflammatory balance

A novel polysaccharide from Polygonatum cyrtonema fibrous root mediates anti-diarrheal effects by reprogramming macrophage M1/M2 polarization

Effect of Puerarin on Chronic Alcoholic Encephalopathy by Modulating the “Microbiota-Gut-Brain Axis” Lipopolysaccharides/Toll-Like Receptors 4/Nuclear Factor Kappa-B Inflammatory Pathway

Ginsenosides Modulate Immunity via TLR4/MyD88/NF-κB Pathway and Gut Microbiota

Integrated network pharmacology, molecular docking and in vivo experiments to elucidate the extenuative mechanisms of ginseng polysaccharide against Toxoplasma gondii-induced testicular toxicity

Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway

Rapamycin Regulates Lipopolysaccharide-Induced Microglial Phagocytosis In Vitro

Dysregulation of Ceramide Metabolism Is Linked to Iron Deposition and Activation of Related Pathways in the Aorta of Atherosclerotic Miniature Pigs

UHPLC-MS-Based Metabolomics Reveal the Potential Mechanism of Armillaria mellea Acid Polysaccharide in and Its Effects on Cyclophosphamide-Induced Immunosuppressed Mice

Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration

An Acidic Polysaccharide with Anti-Inflammatory Effects from Blackened Jujube: Conformation and Rheological Properties

The protective effect of rutin against lipopolysaccharide induced acute lung injury in mice based on the pharmacokinetic and pharmacodynamic combination model

(−)-Epigallocatechin-3-Gallate Protects Against Lithium-Pilocarpine-Induced Epilepsy by Inhibiting the Toll-Like Receptor 4 (TLR4)/Nuclear Factor-κB (NF-κB) Signaling Pathway

Hypoglycemic Mechanism of the Berberine Organic Acid Salt under the Synergistic Effect of Intestinal Flora and Oxidative Stress

Protection of Anthocyanin from Myrica rubra against Cerebral Ischemia-Reperfusion Injury via Modulation of the TLR4/NF-κB and NLRP3 Pathways

Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas After Chronic Alcohol Exposure

Toll-like receptor 4 contributes to chronic itch, alloknesis and spinal astrocyte activation in male mice

Toll-like Receptor 4 Relates to Lipopolysaccharide-induced Mucus Hypersecretion in Rat Airway

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