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Product Info Summary
| SKU: | A30508 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC |
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Product info
Product Name
Anti-TM16A ANO1 Antibody
SKU/Catalog Number
A30508
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TM16A ANO1 Antibody (Catalog# A30508). Tested in ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TM16A ANO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30508)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TM16A. Immunogen sequence location: 921-970
Reactive Species
A30508 is reactive to ANO1 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
114.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30508 is guaranteed for ELISA, IF, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of HeLa cells, using TM16A Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TM16A ANO1 Antibody (A30508)
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Customer Q&As
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4 Customer Q&As for Anti-TM16A ANO1 Antibody
Question
Would anti-TM16A antibody A30508 work for IF with thoracic aorta?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
According to the expression profile of thoracic aorta, ANO1 is highly expressed in thoracic aorta. So, it is likely that anti-TM16A antibody A30508 will work for IF with thoracic aorta.
Boster Scientific Support
Answered: 2020-02-13
Question
Would A30508 anti-TM16A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
It shows on the product datasheet, A30508 anti-TM16A antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-27
Question
Is a blocking peptide available for product anti-TM16A antibody (A30508)?
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
We do provide the blocking peptide for product anti-TM16A antibody (A30508). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-09-03
Question
I see that the anti-TM16A antibody A30508 works with IF, what is the protocol used to produce the result images on the product page?
E. Collins
Verified customer
Asked: 2016-02-18
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-02-18
