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Product Info Summary
| SKU: | M09325 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-TMEM119 Antibody (Monoclonal, 31T39)
SKU/Catalog Number
M09325
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-TMEM119 Antibody (Monoclonal, 31T39) catalog # M09325. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TMEM119 Antibody (Monoclonal, 31T39) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09325)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
31T39
Immunogen
Recombinant protein within mouse TMEM119 aa 115-278.
Reactive Species
M09325 is reactive to TMEM119 in Human, Mouse, Rat
Observed Molecular Weight
52 kDa
Calculated molecular weight
29.2 kDa
Background of TMEM119
Involved in positive regulation of bone mineralization; positive regulation of osteoblast differentiation; and positive regulation of osteoblast proliferation. Located in plasma membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09325 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunocytochemistry/Immunofluorescence, 1:50-200
ImmunoPrecipitation, 1:50
Flow Cytometry (Fixed), 1:50-200
Positive Control
WB: human SH-SY5Y whole cell, human U251 whole cell, rat brain tissue, rat lung tissue, rat C6 whole cell, mouse brain tissue, mouse lung tissue, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
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Western blot analysis of TMEM119 using anti-TMEM119 antibody (M09325).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM119 antigen affinity purified monoclonal antibody (M09325) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TMEM119 at approximately 52 kDa. The expected band size for TMEM119 is at 29 kDa.
Specific Publications For Anti-TMEM119 Antibody (Monoclonal, 31T39) (M09325)
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