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- Table of Contents
TMEM52B antibody (A17417-1) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of RT4 cells using anti-TMEM52B antibody (A17417-1).
Overlay histogram showing RT4 cells stained with A17417-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM52B Antibody (A17417-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing RT4 cells stained with A17417-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM52B Antibody (A17417-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-TMEM52B Antibody Picoband®
TMEM52B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
TMEM52B antibody was positively detected by WB in:
human 293T whole cell, monkey COS-7 whole cell, human SiHa whole cell, rat kidney tissue, rat NRK whole cell, mouse kidney tissue, mouse HBZY-1 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TMEM52B antibody was positively detected by FC in:
RT4 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Monkey, Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-TMEM52B Antibody Picoband®
View all TMEM52B Antibodies
View all TMEM52B Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TMEM52B recombinant protein (Position: T22-N183).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
24 kDa
Calculated Molecular Weight
20.0 kDa
Background of TMEM52B
TMEM52B (Transmembrane Protein 52B, also known as C12orf59) is a Protein Coding gene. The TMEM52B gene, located on 12p13.2, is conserved in chimpanzee, dog, cow, mouse, rat, chicken, and frog. 2 alternatively spliced human isoforms have been reported. The 183-amino acid protein has a reported mass of 20,002 daltons. The cellular localization is predicted to be membrane-associated. C12orf59 might act as a potential oncogenic protein in gastric cancer (GC). Researchers propose that the decreased C12orf59 expression status is a prognostic biomarker of ccRCC and cooperates with the loss of von Hippel-Lindau (VHL) all the while promoting renal carcinogenesis. 192 organisms have orthologs with human gene TMEM52B.
Cite This Product
Anti-TMEM52B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17417-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
93%
High Homology
Dog
87%
Moderate Homology
Mouse
84%
Moderate Homology
Cow
84%
Moderate Homology
Sheep
83%
Moderate Homology
Rat
81%
Moderate Homology
Rabbit
80%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TMEM52B antibody
Flow Cytometry (FC) protocol for TMEM52B antibody
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Product Promise
We promise Anti-TMEM52B Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A17417-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026