Anti-TMEM52B Antibody Picoband™

Product Name

Anti-TMEM52B Antibody Picoband™

View all TMEM52B Antibodies

SKU/Catalog Number

A17417-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TMEM52B Antibody Picoband™ catalog # A17417-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-TMEM52B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17417-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TMEM52B recombinant protein (Position: T22-N183).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A17417-1 is reactive to TMEM52B in Human, Monkey, Mouse, Rat

Applications

A17417-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

24 kDa

Calculated molecular weight

20.002kDa

Background of TMEM52B

TMEM52B (Transmembrane Protein 52B, also known as C12orf59) is a Protein Coding gene. The TMEM52B gene, located on 12p13.2, is conserved in chimpanzee, dog, cow, mouse, rat, chicken, and frog. 2 alternatively spliced human isoforms have been reported. The 183-amino acid protein has a reported mass of 20,002 daltons. The cellular localization is predicted to be membrane-associated. C12orf59 might act as a potential oncogenic protein in gastric cancer (GC). Researchers propose that the decreased C12orf59 expression status is a prognostic biomarker of ccRCC and cooperates with the loss of von Hippel-Lindau (VHL) all the while promoting renal carcinogenesis. 192 organisms have orthologs with human gene TMEM52B.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Monkey, Mouse, Rat
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of TMEM52B using anti-TMEM52B antibody (A17417-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: rat NRK whole cell lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse HBZY-1 whole cell lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM52B antigen affinity purified polyclonal antibody (Catalog # A17417-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM52B at approximately 24 kDa. The expected band size for TMEM52B is at 20 kDa.

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Figure 2. Flow Cytometry analysis of RT4 cells using anti-TMEM52B antibody (A17417-1).
Overlay histogram showing RT4 cells stained with A17417-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM52B Antibody (A17417-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For TMEM52B (Source: Uniprot.org, NCBI)

Alternative Names

C12orf59; chromosome 12 open reading frame 59; UNQ5927/PRO19821 TMEM52B C12orf59 transmembrane protein 52B transmembrane protein 52B

For more info on TMEM52B, check out the TMEM52B Infographic

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In this infographic, you will see the following information for TMEM52B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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