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Product Info Summary
| SKU: | A16616 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-TMIGD1 Antibody
SKU/Catalog Number
A16616
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TMIGD1 Antibody (Catalog # A16616). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TMIGD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-TMIGD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16616)
Host
Rabbit
Contents
TMIGD1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TMIGD1 antibody was raised against an 18 amino acid peptide near the center of human TMIGD1. The immunogen is located within amino acids 150 - 200 of TMIGD1.
Cross-reactivity
TMIGD1 antibody is human, mouse and rat reactive. At least two isoforms of TMIGD1 are known to exist; this antibody will detect both isoforms.
Reactive Species
A16616 is reactive to TMIGD1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
29.2 kDa
Background of TMIGD1
TMIGD1 (transmembrane and immunoglobulin domain containing 1) is a 262 amino acid single-pass membrane protein that contains two Ig-like C2-type (immunoglobulin-like) domains (1,2). It is a nonsecreted, glycosylated protein located in the cytoplasm and cell membrane (1). TMIGD1 might be a possible cell-differentiation marker in the intestine, whose expression is lost during cellular transformation and promote progression of tumorigenesis in the intestinal epithelium (2-4). TMIGD1 has also been postulated to play a role in cell adhesion (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16616 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TMIGD1 antibody can be used for detection of TMIGD1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TMIGD1 in rat liver tissue lysate with TMIGD1 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of TMIGD1 in mouse liver tissue with TMIGD1 antibody at 5 μg/mL.
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Immunofluorescence of TMIGD1 in mouse liver tissue with TMIGD1 antibody at 20 μg/mL.
Specific Publications For Anti-TMIGD1 Antibody (A16616)
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