Product Info Summary
| SKU: | M11415 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-TMIGD2 Monoclonal Antibody [8A1]
SKU/Catalog Number
M11415
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TMIGD2 Monoclonal Antibody [8A1] (Catalog # M11415). Tested in ELISA, WB, ICC, IF, IHC-P, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
TMIGD2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TMIGD2 Monoclonal Antibody [8A1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11415)
Host
Mouse
Contents
TMIGD2 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Clonality
Monoclonal
Clone Number
Clone: 8A1
Isotype
IgG1,k
Immunogen
TMIGD2 antibody was raised against the extracellular domain of human TMIGD2
Reactive Species
M11415 is reactive to TMIGD2 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
30.7 kDa
Background of TMIGD2
TMIGD2 (transmembrane and immunoglobulin domain containing 1), also known as immunoglobulin-containing and proline-rich receptor 1 (IGPR1), is novel adhesion molecule that is expressed in multiple tissues, primarily in cells of epithelium and endothelium origins (1). TMIGD2 is thought to be involved in angiogenesis and regulates cellular morphology, homophilic cell aggregation, and cell-cell interaction. TMIGD2 activity also modulates actin stress fiber formation and focal adhesion and reduces cell migration.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11415 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TMIGD2 antibody can be used for ELISA starting at 0.25 μg/mL. For Western blot start at 0.25 μg/mL. For Immunocytochemistry start at 2 μg/mL. For Immunofluorescence start at 20 μg/mL. For Immunohistochemistry start at 5 μg/mL. For Flow Cytometry start at 10 μg/ml. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TMIGD2 in overexpressing HEK293 cells TMIGD2 antibody at 0.25, 0.5, and 1 μg/ml
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Immunocytochemistry of TMIGD2 in transfected HEK293 cells with TMIGD2 antibody at 2 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 2 μg/mL.
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Immunofluorescence of TMIGD2 in human colon carcinoma tissue with TMIGD2 antibody at 20 μg/mL.
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Immunohistochemistry of TMIGD2 in human colon carcinoma tissue with TMIGD2 antibody at 5 μg/mL.
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Flow cytometry analysis of TMIGD2 overexpressing HEK293 cells using TMIGD2 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: TIM-3 overexpressing HEK293 cells.
Specific Publications For Anti-TMIGD2 Monoclonal Antibody [8A1] (M11415)
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