Anti-TNF-Alpha (Q178) Antibody

TNF-alpha antibody

Boster Bio Anti-TNF-Alpha (Q178) Antibody catalog # A00002. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat. Cited in 10 publication(s).

Product Info Summary

SKU: A00002
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, WB

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Product Name

Anti-TNF-Alpha (Q178) Antibody

View all TNF-alpha Antibodies

SKU/Catalog Number

A00002

Size

100ul

Form

Liquid

Description

Boster Bio Anti-TNF-Alpha (Q178) Antibody catalog # A00002. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TNF-Alpha (Q178) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00002)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Clone Number

PI9-17

Isotype

IgG

Immunogen

Synthesized peptide derived from human p53 around the phosphorylation site of T81.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Weakly cross-reacts with dog p53.

Reactive Species

A00002 is reactive to TNF in Human, Mouse, Rat

Applications

A00002 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

25.644kDa

Background of TNF-alpha

Anti-Glycogen Synthase 1 pS641 antibody is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3 that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S641. Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:200

Validation Images & Assay Conditions

Gene/Protein Information For TNF (Source: Uniprot.org, NCBI)

Gene Name

TNF

Full Name

Tumor necrosis factor

Weight

25.644kDa

Superfamily

Tumor necrosis factor family

Alternative Names

APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha TNF DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF tumor necrosis factor tumor necrosis factor|APC1 protein|TNF, macrophage-derived|TNF, monocyte-derived|TNF-a|tumor necrosis factor ligand 1F|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TNF, check out the TNF Infographic

TNF infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TNF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00002 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Novel Multifunctional Silver Nanocomposite Serves as a Resistance-Reversal Agent to Synergistically Combat Carbapenem-Resistant Acinetobacter baumannii

Nifedipine inhibits oxidative stress and ameliorates osteoarthritis by activating the nuclear factor erythroid-2-related factor 2 pathway

Effects of Ultrashort Wave Therapy on Inflammation and Macrophage Polarization after Acute Lung Injury in Rats

Effects of Ultrashort Wave Therapy on Inflammation and Macrophage Polarization after Acute Lung Injury in Rats

Effects of Ultrashort Wave Therapy on Inflammation and Macrophage Polarization after Acute Lung Injury in Rats

Sun S,Wang Y,Du Y,Sun Q,He L,Zhu E,Li J. Oxidative stress-mediated hepatotoxicity in rats induced by ethanol extracts of different parts of Chloranthus serratus.Pharm Biol.2020 Dec;58(1):1277-1289.doi:10.1080/ n13880209.2020.1859552.PMID: 33355514.
Species: Rat
A00002 usage in article: APP:IHC, SAMPLE:LIVER TISSUE, DILUTION:NA

Guo Y,Gu R,Yu J,Lei B,Gan D,Xu G.Synthetic Glucocorticoid-Induced Leucine Zipper Peptide Inhibits Lipopolysaccharide-Induced Ocular Inflammation in Rats.Ophthalmic Res.2020;63(4):434-442.doi:10.1159/000505 003.Epub 2019 Nov 26.PMID:31770752.
Species: Rat
A00002 usage in article: APP:WB, SAMPLE:RETINAL TISSUE, DILUTION:1:500

Qi MY,Wang XT,Xu HL,Yang ZL,Cheng Y,Zhou B.Protective effect of ferulic acid on STZ-induced diabetic nephropathy in rats.Food Funct.2020 Apr 1;11(4):3706-3718.doi:10.1039/c9fo02398d.Epub 2020 Apr 20. PMID:32307498.
Species: Rat
A00002 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:1000

Zhao B,Li S,Guo Z,Chen Z,Zhang X,Xu C,Chen J,Wei C. Dopamine receptor D2 inhibition alleviates diabetic hepatic stellate cells fibrosis by regulating the TGF-β1/Smads and NFκB pathways. Clin Exp Pharmacol Physiol.2020 Nov 11.doi:10.1111/1440-1681.13437.Ep
Species: Rat
A00002 usage in article: APP:WB, SAMPLE:LIVER TISSUE AND HSCS, DILUTION:NA

Huang CL,Liu F,Zhang YY,Lin J,Fu M,Li YL, Zhou C,Li CJ,Shen JF. Activation of oxytocin receptor in the trigeminal ganglion attenuates orofacial ectopic pain attributed to inferior alveolar nerve injury. J Neurophysiol.2020 Dec 16.doi:10.1152/jn.00646. 202
Species: Rat
A00002 usage in article: APP:WB, SAMPLE:TG CELL AND SPVC CELL, DILUTION:1:1000

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Order DetailsPrice
A00002-100ul

100ul

$405

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