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Product Info Summary
| SKU: | PA1361 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TNF beta/LTA Antibody Picoband®
SKU/Catalog Number
PA1361
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TNF beta/LTA Antibody catalog # PA1361. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TNF beta/LTA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1361)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TNF beta, different from the related mouse sequence by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1361 is reactive to LTA in Human
Observed Molecular Weight
6 kDa
Calculated molecular weight
22.3 kDa
Background of LTA
Lymphotoxin (previously known as tumor necrosis factor-beta) is a lymphokine cytokine. It is a protein that is produced by Th1 type T-cells and induces vascular endothelial cells to change their surface adhesion molecules to allow phagocytic cells to bind to them. Lymphotoxin is homologous to Tumor Necrosis Factor beta, but secreted by T-cells. It is paracrine due to the small amounts produced. The effects are similar to TNF-alpha, but TNF-beta is also important for the development of lymphoid organs. Nedwin et al. (1985) found that TNFA and TNFB are closely linked on chromosome 6. Study of hybrid cells made with rearranged human chromosome 6 showed that both TNFA and TNFB map to the 6p23-q12 segment.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1361 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-TNF beta antibody, PA1361, Western blotting
Lane 1: Recombinant Human TNF beta Protein 10ng
Lane 2: Recombinant Human TNF beta Protein 5ng
Lane 3: Recombinant Human TNF beta Protein 2.5ng
Specific Publications For Anti-TNF beta/LTA Antibody Picoband® (PA1361)
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Customer Q&As
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7 Customer Q&As for Anti-TNF beta/LTA Antibody Picoband®
Question
I was wanting to use your anti-TNF beta/LTA antibody for WB for human lymph on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lymph identification?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
It shows on the product datasheet, PA1361 anti-TNF beta/LTA antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymph in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-04
Question
My question regarding product PA1361, anti-TNF beta/LTA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1361 anti-TNF beta/LTA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-24
Question
Would anti-TNF beta/LTA antibody PA1361 work on monkey WB with lymph?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
Our lab technicians have not validated anti-TNF beta/LTA antibody PA1361 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-TNF beta/LTA antibody PA1361 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey lymph in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-16
Question
I see that the anti-TNF beta/LTA antibody PA1361 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-01
Question
Do you have a BSA free version of anti-TNF beta/LTA antibody PA1361 available?
Verified Customer
Verified customer
Asked: 2019-01-22
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-TNF beta/LTA antibody PA1361 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-22
Question
I am interested in to test anti-TNF beta/LTA antibody PA1361 on human lymph for research purposes, then I may be interested in using anti-TNF beta/LTA antibody PA1361 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-04-11
Answer
The products we sell, including anti-TNF beta/LTA antibody PA1361, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-11
Question
Is this PA1361 anti-TNF beta/LTA antibody reactive to the isotypes of LTA?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
The immunogen of PA1361 anti-TNF beta/LTA antibody is A synthetic peptide corresponding to a sequence in the middle region of human TNF beta(73-88aa KQNSLLWRANTDRAFL), different from the related mouse sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-12
