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Product Info Summary
| SKU: | A30476 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-TNFC LTB Antibody
SKU/Catalog Number
A30476
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TNFC LTB Antibody (Catalog# A30476). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TNFC LTB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30476)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TNFC. Immunogen sequence location: 181-230
Reactive Species
A30476 is reactive to LTB in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
25.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30476 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of various cells using LT-β Polyclonal Antibody
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Immunofluorescence analysis of HeLa cells, using TNFC Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HepG2 cells, using TNFC Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TNFC LTB Antibody (A30476)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-TNFC LTB Antibody
Question
Does A30476 anti-TNFC antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
As indicated on the product datasheet, A30476 anti-TNFC antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-03
Question
We are currently using anti-TNFC antibody A30476 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
The anti-TNFC antibody (A30476) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-04
Question
See attached the WB image, lot number and protocol we used for spleen using anti-TNFC antibody A30476. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-27
Question
I was wanting to use your anti-TNFC antibody for WB for human spleen on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human spleen identification?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
As indicated on the product datasheet, A30476 anti-TNFC antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-12
Question
Do you have a BSA free version of anti-TNFC antibody A30476 available?
Verified Customer
Verified customer
Asked: 2018-08-31
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TNFC antibody A30476 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-31
Question
Would anti-TNFC antibody A30476 work on dog WB with colon?
L. Mitchell
Verified customer
Asked: 2014-06-04
Answer
Our lab technicians have not tested anti-TNFC antibody A30476 on dog. You can run a BLAST between dog and the immunogen sequence of anti-TNFC antibody A30476 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog colon in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-06-04
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for spleen using anti-TNFC antibody A30476. Let me know if you need anything else.
K. Carter
Verified customer
Asked: 2013-09-11
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-09-11
