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Product Info Summary
| SKU: | M04143 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-TOM1 Antibody (Monoclonal, 32T73)
SKU/Catalog Number
M04143
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-TOM1 Antibody (Monoclonal, 32T73) catalog # M04143. Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TOM1 Antibody (Monoclonal, 32T73) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04143)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
32T73
Immunogen
Recombinant protein within human TOM1 aa 302-448.
Reactive Species
M04143 is reactive to TOM1 in Human, Mouse, Rat
Observed Molecular Weight
54 kDa
Calculated molecular weight
53.8 kDa
Background of TOM1
Target of Myb protein 1 is a protein that in humans is encoded by the TOM1 gene. This gene was identified as a target of the v-myb oncogene. The encoded protein shares its N-terminal domain in common with proteins associated with vesicular trafficking at the endosome. It is recruited to the endosomes by its interaction with endofin. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04143 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Immunofluorescence, 1:50-200
Positive Control
WB: human HepG2 whole cell lysates, human SIHA whole cell lysates, human U2OS whole cell lysates, human Caco-2 whole cell lysates, rat brain tissue lysates, rat heart tissue lysates, mouse brain tissue lysates, mouse heart tissue lysates
Validation Images & Assay Conditions
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Western blot analysis of TOM1 using anti-TOM1 antibody (M04143).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human SIHA whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOM1 antigen affinity purified monoclonal antibody (M04143) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TOM1 at approximately 54 kDa. The expected band size for TOM1 is at 54 kDa.
Specific Publications For Anti-TOM1 Antibody (Monoclonal, 32T73) (M04143)
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