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- Table of Contents
TOM1L1 antibody (A07598-2) performs well in ELISA, Flow Cytometry, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of JK cells using anti-TOM1L1 antibody (A07598-2).
Overlay histogram showing JK cells stained with A07598-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOM1L1 Antibody (A07598-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing JK cells stained with A07598-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOM1L1 Antibody (A07598-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing an IHC experiment for TOM1L1? The guide covers the expected staining pattern, antigen retrieval, antibodies backed by real IHC images, tissue controls, and protocols taken from published papers.
Open the TOM1L1 IHC GuideBundle Save
Anti-TOM1L1 Antibody Picoband®
TOM1L1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, WB |
Tested Applications
TOM1L1 antibody was positively detected by WB in:
human Hela whole cell, human 293T whole cell, human T-47D whole cell, human HEL whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TOM1L1 antibody was positively detected by IHC in:
human colon cancer tissue, human liver cancer tissue, human lung cancer tissue, human thyroid cancer tissue, human prostate adenocarcinoma tissue, mouse brain tissue, rat brain tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TOM1L1 antibody was positively detected by IF in:
human rectal cancer tissue, human ovarian cancer tissue
TOM1L1 antibody was positively detected by FC in:
JK cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Mouse
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-TOM1L1 Antibody Picoband®
View all TOM1L1 Antibodies
View all TOM1L1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TOM1L1 recombinant protein (Position: D9-D448).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
53.0 kDa
Background of TOM1L1
TOM1-like protein 1 is a protein that in humans is encoded by the TOM1L1 gene. Enables clathrin binding activity and protein kinase binding activity. Involved in negative regulation of mitotic nuclear division; positive regulation of protein phosphorylation; and signal transduction. Located in cytosol and endosome.
Cite This Product
Anti-TOM1L1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07598-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Dog
86%
Moderate Homology
Rabbit
85%
Moderate Homology
Cow
85%
Moderate Homology
Sheep
85%
Moderate Homology
Mouse
82%
Moderate Homology
Rat
72%
Moderate Homology
Drosophila
33%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TOM1L1 antibody
Immunohistochemistry (IHC) protocol for TOM1L1 antibody
Immunofluorescence (IF) protocol for TOM1L1 antibody
Flow Cytometry (FC) protocol for TOM1L1 antibody
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Product Promise
We promise Anti-TOM1L1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A07598-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 16, 2026