Product Info Summary
SKU: | A02750-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Topo II Beta (A32) TOP2B Antibody
SKU/Catalog Number
A02750-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Topo II Beta (A32) TOP2B Antibody catalog # A02750-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Topo II Beta (A32) TOP2B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02750-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human POLDIP3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02750-1 is reactive to TOP2B in Human, Mouse, Rat
Applications
A02750-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
183.267kDa
Background of TOP2B
Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules.
Burd C.G., Proc. Natl. Acad. Sci. U.S.A. 86:9788-9792(1989).
Swanson M.S., Mol. Cell. Biol. 7:1731-1739(1987).
Merrill B.M., Nucleic Acids Res. 17:8441-8449(1989).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Topo II Beta (A32) TOP2B Antibody A02750-1
Western blot (WB) analysis of Topo IIβ (A32) pAb at 1:500 dilutionLane1:A549 whole cell lysate(20ug)
Lane2:HEK293T whole cell lysate(20ug)
Lane3:AML-12 whole cell lysate(20ug)
Lane4:PC12 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Figure 2. Immunohistochemistry validation of TOP2B using Anti-Topo II Beta (A32) TOP2B Antibody (A02750-1).
Immunohistochemistry (IHC) analyzes of Topo IIβ (A32) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.For more protocol information of IHC
Protein Target Info & Infographic
Gene/Protein Information For TOP2B (Source: Uniprot.org, NCBI)
Gene Name
TOP2B
Full Name
DNA topoisomerase 2-beta
Weight
183.267kDa
Superfamily
type II topoisomerase family
Alternative Names
antigen MLAA-44; DNA topoisomerase 2-beta; DNA topoisomerase II beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; EC 5.99.1.3; TOP2B; top2beta; TOPIIB; topo II beta; topoisomerase (DNA) II beta (180kD); topoisomerase (DNA) II beta 180kDa; topoisomerase II beta; topoisomerase IIb; U937 associated antigen TOP2B TOPIIB, top2beta DNA topoisomerase II beta DNA topoisomerase 2-beta|DNA topoisomerase II, 180 kD|DNA topoisomerase II, beta isozyme|U937 associated antigen|antigen MLAA-44|topo II beta|topoisomerase (DNA) II beta 180kDa|topoisomerase II beta|topoisomerase IIb
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOP2B, check out the TOP2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOP2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Topo II Beta (A32) TOP2B Antibody (A02750-1)
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No publications found for A02750-1
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