DNA topoisomerase 2-beta Antibodies

AND TOP2B ELISA kits, DNA topoisomerase 2-beta Proteins

Many biological assays use antibodies to detect DNA topoisomerase 2-beta, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with DNA topoisomerase 2-beta in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop DNA topoisomerase 2-beta antibodies...

We validate the specificity of these antibodies to DNA topoisomerase 2-beta by testing them on tissues known to express TOP2B positively and negatively. Browse below to find the TOP2B antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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TOP2B Infographic by Boster Bio

All TOP2B Products

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Anti-TOP2B/Topoisomerase Ii Beta Antibody

4 Q&As

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TOP2B Antibody

Western blot analysis of lysates from Jurkat cells, using TOP2B Antibody

Human, Mouse

ELISA, IF, IHC, ICC, WB

$335
Cat # A02750
100ul

TOP2B Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from Jurkat cells

Human, Mouse

ELISA

$480
Cat # EKC1572
1 kit, containing one 96-well plate and all necessary reagents

Anti-Topo II Beta (A32) TOP2B Antibody

Western blot (WB) analysis of Topo IIβ (A32) pAb at 1:500 dilution
Lane1:A549 whole cell lysate(20ug)
Lane2:HEK293T whole cell lysate(20ug)
Lane3:AML-12 whole cell lysate(20ug)
Lane4:PC12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Topo IIβ (A32) pAb in paraffin-embedded human lung carcinoma tissue at 1:100

Human, Mouse, Rat

IHC, WB

$405
Cat # A02750-1
100ul

Anti-TOP2B Antibody

Human, Mouse

ELISA, IP, IF, IHC, ICC, WB

$370
Cat # A02750-2
100 μl/vial

TOP2B Overview

Facts about DNA topoisomerase 2-beta.

TOP2B 3D structure. Source: alphafold

DNA topoisomerase 2-beta (TOP2B)

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.

Gene Information

Human
Gene Name:	TOP2B
Uniprot:	Q02880
Entrez:	7155

Family

Belongs to:

type II topoisomerase family

Alternative Names

antigen MLAA-44; DNA topoisomerase 2-beta; DNA topoisomerase II beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; EC 5.99.1.3; TOP2B; top2beta; TOPIIB; topo II beta; topoisomerase (DNA) II beta (180kD); topoisomerase (DNA) II beta 180kDa; topoisomerase II beta; topoisomerase IIb; U937 associated antigen

Molecular Weight

Mass (kDA):
183.267 kDA

Genomic Context


Human

Location:	3p24.2
Sequence:	3; NC_000003.12 (25597905..25664891, complement)

Subcellular Localizations

Cytoplasm. Nucleus, nucleolus.

Diseases

Malignant Neoplasms
Leukemia
Carcinoma
Neoplasms
Myeloid Leukemia
Leukemia, Myelocytic, Acute
Liver Carcinoma
Heart Failure
Malignant Paraganglionic Neoplasm
Cardiotoxicity
Chromosomal Translocation
Liver Neoplasms
Malignant Neoplasm Of Prostate

Neoplasm Metastasis
Intraepithelial Neoplasia
Malignant Neoplasm Of Breast
Glioma
Fibrosis
Acute Promyelocytic Leukemia
Therapy-related Leukemia

Pathways

Fertilization
Dna Repair
Drug Resistance
Dna Replication
Cell Cycle
Chromosome Segregation
Methylation
Reverse Transcription
Chromosome Condensation
Chromatin Remodeling
Transport
Cell Adhesion
Regulation Of Cell Cycle

Mitosis
Immune Response
Dna Methylation
Protein Transport
Cell Division
Error-prone Dna Repair
Translation

PTMs

Cleavage

Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TOP2B

References

PMID: 1333583 by Jenkins J.R., et al. Isolation of cDNA clones encoding the beta isozyme of human DNA topoisomerase II and localisation of the gene to chromosome 3p24.

PMID: 8383537 by Austin C.A., et al. Novel HeLa topoisomerase II is the II beta isoform: complete coding sequence and homology with other type II topoisomerases.