Click image to see more details
Product Info Summary
| SKU: | A02750 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TOP2B/Topoisomerase Ii Beta Antibody
SKU/Catalog Number
A02750
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TOP2B/Topoisomerase Ii Beta Antibody (Catalog# A02750). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TOP2B/Topoisomerase Ii Beta Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02750)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TOP2B. Immunogen sequence location: 1-50
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02750 is reactive to TOP2B in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
183.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02750 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TOP2B Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat cells, using TOP2B Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TOP2B/Topoisomerase Ii Beta Antibody (A02750)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-TOP2B/Topoisomerase Ii Beta Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-TOP2B/Topoisomerase Ii Beta antibody A02750. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-18
Question
Would anti-TOP2B/Topoisomerase Ii Beta antibody A02750 work for IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
According to the expression profile of leukemic t-cell, TOP2B is highly expressed in leukemic t-cell. So, it is likely that anti-TOP2B/Topoisomerase Ii Beta antibody A02750 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2019-12-16
Question
Please see the WB image, lot number and protocol we used for leukemic t-cell using anti-TOP2B/Topoisomerase Ii Beta antibody A02750. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-12
Question
We are currently using anti-TOP2B/Topoisomerase Ii Beta antibody A02750 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
The anti-TOP2B/Topoisomerase Ii Beta antibody (A02750) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-08
