Anti-TOP2B/Topoisomerase Ii Beta Antibody

TOP2B antibody

Boster Bio Anti-TOP2B/Topoisomerase Ii Beta Antibody (Catalog# A02750). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A02750
Size: 100ul
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, IHC-P, WB, IF (paraffin)

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Product Name

Anti-TOP2B/Topoisomerase Ii Beta Antibody

View all TOP2B Antibodies

SKU/Catalog Number

A02750

Size

100ul

Form

Liquid

Description

Boster Bio Anti-TOP2B/Topoisomerase Ii Beta Antibody (Catalog# A02750). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TOP2B/Topoisomerase Ii Beta Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02750)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

7A7

Isotype

Rabbit IgG

Immunogen

A peptide derived from human TOP2B. Immunogen sequence location: 1-50

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A02750 is reactive to TOP2B in Human, Mouse

Applications

A02750 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

183.267kDa

Background of TOP2B

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For TOP2B (Source: Uniprot.org, NCBI)

Gene Name

TOP2B

Full Name

DNA topoisomerase 2-beta

Weight

183.267kDa

Superfamily

type II topoisomerase family

Alternative Names

antigen MLAA-44; DNA topoisomerase 2-beta; DNA topoisomerase II beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; EC 5.99.1.3; TOP2B; top2beta; TOPIIB; topo II beta; topoisomerase (DNA) II beta (180kD); topoisomerase (DNA) II beta 180kDa; topoisomerase II beta; topoisomerase IIb; U937 associated antigen TOP2B TOPIIB, top2beta DNA topoisomerase II beta DNA topoisomerase 2-beta|DNA topoisomerase II, 180 kD|DNA topoisomerase II, beta isozyme|U937 associated antigen|antigen MLAA-44|topo II beta|topoisomerase (DNA) II beta 180kDa|topoisomerase II beta|topoisomerase IIb

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TOP2B, check out the TOP2B Infographic

TOP2B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TOP2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-TOP2B/Topoisomerase Ii Beta Antibody

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-TOP2B/Topoisomerase Ii Beta antibody A02750. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-18

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-18

Question

Would anti-TOP2B/Topoisomerase Ii Beta antibody A02750 work for IHC with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

According to the expression profile of leukemic t-cell, TOP2B is highly expressed in leukemic t-cell. So, it is likely that anti-TOP2B/Topoisomerase Ii Beta antibody A02750 will work for IHC with leukemic t-cell.

Boster Scientific Support

Answered: 2019-12-16

Question

Please see the WB image, lot number and protocol we used for leukemic t-cell using anti-TOP2B/Topoisomerase Ii Beta antibody A02750. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-11-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-12

Question

We are currently using anti-TOP2B/Topoisomerase Ii Beta antibody A02750 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2017-08-08

Answer

The anti-TOP2B/Topoisomerase Ii Beta antibody (A02750) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-08-08

Order DetailsPrice
A02750

100 μL

$335

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