Anti-TPMT Antibody Picoband™

TPMT antibody

Boster Bio Anti-TPMT Antibody Picoband™ catalog # A00671-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00671-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-TPMT Antibody Picoband™

View all TPMT Antibodies

SKU/Catalog Number

A00671-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TPMT Antibody Picoband™ catalog # A00671-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TPMT Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00671-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human TPMT recombinant protein (Position: M1-K245).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00671-1 is reactive to TPMT in Human, Mouse, Rat

Applications

A00671-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

28.18kDa

Background of TPMT

Thiopurine methyltransferase or thiopurine S-methyltransferase (TPMT) is an enzyme that in humans is encoded by the TPMT gene. This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For TPMT (Source: Uniprot.org, NCBI)

Gene Name

TPMT

Full Name

Thiopurine S-methyltransferase

Weight

28.18kDa

Superfamily

class I-like SAM-binding methyltransferase superfamily

Alternative Names

EC 2.1.1.67; S-adenosyl-L-methionine:thiopurine S-methyltransferase; Thiopurine methyltransferase; thiopurine S-methyltransferase TPMT TPMTD thiopurine S-methyltransferase thiopurine S-methyltransferase|S-adenosyl-L-methionine:thiopurine S-methyltransferase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TPMT, check out the TPMT Infographic

TPMT infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TPMT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A00671-1

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-TPMT Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-TPMT Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

5 Customer Q&As for Anti-TPMT Antibody Picoband™

Question

Would anti-TPMT antibody A00671-1 work on dog ELISA with blood?

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

Our lab technicians have not validated anti-TPMT antibody A00671-1 on dog. You can run a BLAST between dog and the immunogen sequence of anti-TPMT antibody A00671-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog blood in ELISA, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-10

Question

We are currently using anti-TPMT antibody A00671-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-17

Answer

The anti-TPMT antibody (A00671-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-17

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-TPMT antibody A00671-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-13

Question

Will anti-TPMT antibody A00671-1 work for WB with bone marrow?

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

According to the expression profile of bone marrow, TPMT is highly expressed in bone marrow. So, it is likely that anti-TPMT antibody A00671-1 will work for WB with bone marrow.

Boster Scientific Support

Answered: 2019-08-07

Question

Is this A00671-1 anti-TPMT antibody reactive to the isotypes of TPMT?

F. Miller

Verified customer

Asked: 2018-06-25

Answer

The immunogen of A00671-1 anti-TPMT antibody is E. coli-derived human TPMT recombinant protein (Position: M1-K245). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-06-25

Order DetailsPrice
A00671-1

100μg

$370
A00671-1-10ug

10μg sample (liquid)

$99
A00671-1-Biotin

100 μg Biotin conjugated

$570
A00671-1-Cy3

100 μg Cy3 conjugated

$570
A00671-1-Dylight488

100 μg Dylight488 conjugated

$570
A00671-1-Dylight550

100 μg Dylight550 conjugated

$570
A00671-1-Dylight594

100 μg Dylight594 conjugated

$570
A00671-1-FITC

100 μg FITC conjugated

$570
A00671-1-HRP

100 μg HRP conjugated

$570
A00671-1-APC

100 μg APC conjugated

$670
A00671-1-PE

100 μg PE conjugated

$670
A00671-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
A00671-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.