TPMT Antibodies

AND TPMT ELISA kits, TPMT Proteins

Many biological assays use antibodies to detect TPMT, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with TPMT in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop TPMT antibodies...

We validate the specificity of these antibodies to TPMT by testing them on tissues known to express TPMT positively and negatively. Browse below to find the TPMT antibody that suites your experiment. We have 6 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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TPMT Infographic by Boster Bio

All TPMT Products

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Anti-TPMT Antibody Picoband®

5 Q&As

Western blot analysis of TPMT using anti-TPMT antibody (A00671-1)

Human, Mouse, Rat

ELISA, WB

$370
Cat # A00671-1
100 μg/vial

TPMT (NM_000367) Human Recombinant Protein

Coomassie blue staining of purified TPMT protein

$1249
Cat # PROTP51580
20 µg

Anti-TPMT Mouse Monoclonal Antibody [Clone ID: OTI2A2]

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPMT (Right lane) cDNA for 48 hrs and lysed

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-TPMT mouse monoclonal antibody

Human

IF, IHC, WB

$523
Cat # M00671
100 µl

Anti-TPMT Mouse Monoclonal Antibody [Clone ID: OTI4C1]

WB analysis of extracts (35ug) from 9 different cell lines by using anti-TPMT monoclonal antibody

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-PIM2 mouse monoclonal antibody

Dog, Human

Flow Cytometry, IHC, WB

$523
Cat # M00671-1
100 µl

Anti-TPMT Rabbit Monoclonal Antibody

Western blot analysis of TPMT expression in Jurkat cell lysate

Human, Mouse, Rat

Flow Cytometry, IHC, WB

$370
Cat # M00671-2
100 μl/vial

TPMT (NM_000367) Human Over-expression Lysate

Human

$628
Cat # LS007172
20 µg, 100 µg

TPMT Overview

Facts about Thiopurine S-methyltransferase.

TPMT 3D structure. Source: alphafold

Thiopurine S-methyltransferase (TPMT)

Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine (also referred to as mercaptopurine, 6-MP or its brand name Purinethol) and 6-thioguanine (also referred to as tioguanine or 6- TG) using S-adenosyl-L-methionine as the methyl donor (PubMed:657528, PubMed:18484748). TPMT activity modulates the cytotoxic effects of thiopurine prodrugs.

A natural substrate for this enzyme has not yet been identified. .

Gene Information

Human
Gene Name:	TPMT
Uniprot:	P51580
Entrez:	7172

Family

Belongs to:

class I-like SAM-binding methyltransferase superfamily

Alternative Names

EC 2.1.1.67; S-adenosyl-L-methionine:thiopurine S-methyltransferase; Thiopurine methyltransferase; thiopurine S-methyltransferase

Molecular Weight

Mass (kDA):
28.18 kDA

Genomic Context


Human

Location:	6p22.3
Sequence:	6; NC_000006.12 (18128311..18155169, complement)

Subcellular Localizations

Cytoplasm.

Diseases

Inflammatory Bowel Diseases
Leukemia
Intestinal Diseases
Acute Lymphocytic Leukemia
Crohn Disease
Thiopurine S Methyltranferase Deficiency
Leukopenia
Malignant Neoplasms
Ulcerative Colitis
Medication Reaction
Autoimmune Reaction
Neoplasms

Colitis
Ulcer
Hepatotoxicity
Autoimmune Diseases
Pancytopenia
L1 Acute Lymphoblastic Leukemia
Rheumatoid Arthritis

Pathways

Methylation
Transport
Translation
Conjugation
Proteolysis
Hypersensitivity
Excretion
Autophagy
Drug Transport
Pathogenesis
Programmed Cell Death
Defecation Rhythm
Dna Repair

Dna Methylation
Response To Drug
Cell Division
Drug Resistance
Dna Amplification
Fermentation
Cell Cycle

PTMs

Methylation
Reduction
Phosphorylation

Cleavage
Acetylation
Oxidation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TPMT

References

PMID: 8316220 by Honchel R., et al. Human thiopurine methyltransferase: molecular cloning and expression of T84 colon carcinoma cell cDNA.

PMID: 7628307 by Lee D., et al. Thiopurine methyltransferase pharmacogenetics. Cloning of human liver cDNA and a processed pseudogene on human chromosome 18q21.1.