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Product Info Summary
| SKU: | M00320 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-TPO Monoclonal Antibody
SKU/Catalog Number
M00320
BM5752 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TPO Monoclonal Antibody catalog # M00320. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TPO Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00320)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFFI-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TPO Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T (3) and T (4) .
Reactive Species
M00320 is reactive to TPO in Human
Observed Molecular Weight
103 kDa
Calculated molecular weight
103.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00320 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:40
Positive Control
WB: Human thyroid
IHC: human thyroid tissue, human thyroid cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of TPO expression in Human thyroid lysate.
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IHC analysis of TPO using anti-TPO antibody (M00320).
TPO was detected in a paraffin-embedded section of human thyroid tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TPO Antibody (M00320) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of TPO using anti-TPO antibody (M00320).
TPO was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TPO Antibody (M00320) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-TPO Monoclonal Antibody (M00320)
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4 Customer Q&As for Anti-TPO Monoclonal Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of thyroid gland using anti-TPO Monoclonal antibody M00320. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-25
Question
Is this M00320 anti-TPO Monoclonal antibody reactive to the isotypes of TPO?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
The immunogen of M00320 anti-TPO Monoclonal antibody is A synthesized peptide derived from human TPO Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T (3) and T (4) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-18
Question
I have attached the WB image, lot number and protocol we used for right lobe of thyroid gland using anti-TPO Monoclonal antibody M00320. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-17
Question
I see that the anti-TPO Monoclonal antibody M00320 works with WB, what is the protocol used to produce the result images on the product page?
P. Miller
Verified customer
Asked: 2017-03-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-03-09
