Anti-TPO Monoclonal Antibody

Thyroid Peroxidase antibody

Boster Bio Anti-TPO Monoclonal Antibody catalog # M00320. Tested in WB, IHC, IP applications. This antibody reacts with Human.

Product Info Summary

SKU: M00320
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IP, IHC, WB

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Product Name

Anti-TPO Monoclonal Antibody

View all Thyroid Peroxidase Antibodies

SKU/Catalog Number

M00320

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-TPO Monoclonal Antibody catalog # M00320. Tested in WB, IHC, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TPO Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00320)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AFFI-20

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human TPO Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T (3) and T (4) .

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00320 is reactive to TPO in Human

Applications

M00320 is guaranteed for IP, IHC, WB Boster Guarantee

Observed Molecular Weight

84 kDa

Calculated molecular weight

102.963kDa

Background of Thyroid Peroxidase

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
IP 1:40

Validation Images & Assay Conditions

Gene/Protein Information For TPO (Source: Uniprot.org, NCBI)

Gene Name

TPO

Full Name

Thyroid peroxidase

Weight

102.963kDa

Superfamily

peroxidase family

Alternative Names

EC 1.11.1; MSA; TDH2A; thyroid microsomal antigen; thyroid peroxidase; thyroperoxidase; TPXEC 1.11.1.8 TPO MSA, TDH2A, TPX thyroid peroxidase thyroid peroxidase|thyroid microsomal antigen|thyroperoxidase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TPO, check out the TPO Infographic

TPO infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TPO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-TPO Monoclonal Antibody

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of thyroid gland using anti-TPO Monoclonal antibody M00320. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-25

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-25

Question

Is this M00320 anti-TPO Monoclonal antibody reactive to the isotypes of TPO?

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

The immunogen of M00320 anti-TPO Monoclonal antibody is A synthesized peptide derived from human TPO Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T (3) and T (4) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-18

Question

I have attached the WB image, lot number and protocol we used for right lobe of thyroid gland using anti-TPO Monoclonal antibody M00320. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-12-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-12-17

Question

I see that the anti-TPO Monoclonal antibody M00320 works with WB, what is the protocol used to produce the result images on the product page?

P. Miller

Verified customer

Asked: 2017-03-09

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-03-09

Order DetailsPrice
M00320

100μL

$370
M00320-30ul

30μL sample

$99
M00320-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M00320
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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