Product Info Summary
SKU: | A01365 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-TRAF1 Antibody Picoband™
SKU/Catalog Number
A01365
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRAF1 Antibody Picoband™ catalog # A01365. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAF1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01365)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRAF1 recombinant protein (Position: D14-D404).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01365 is reactive to TRAF1 in Human
Applications
A01365 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
46 kDa
Calculated molecular weight
46.164kDa
Background of TRAF-1
TRAF1 (TNF Receptor-Associated Factor 1), also called EBI6, is a protein that in humans is encoded by the TRAF1 gene. The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. Siemienski et al. (1997) used fluorescence in situ hybridization to map the TRAF1 gene to 9q33-q34. Mosialos et al. (1995) found that LMP1, the EBV-transforming protein, specifically associates with LAP1 (TRAF3) or EBI6 in B lymphoblasts. LMP1 expression redirects LAP1 and EBI6 from scattered cytoplasmic structures to LMP1 plasma membrane patches. Both LAP1 and EBI6 associated with the cytoplasmic domain of p80/TNFR2 in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TRAF1 using anti-TRAF1 antibody (A01365).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HELA whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: human HEPG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF1 antigen affinity purified polyclonal antibody (Catalog # A01365) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF1 at approximately 46KD. The expected band size for TRAF1 is at 46KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of A549 cells using anti-TRAF1 antibody (A01365).
Overlay histogram showing A549 cells stained with A01365 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAF1 Antibody (A01365, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For TRAF1 (Source: Uniprot.org, NCBI)
Gene Name
TRAF1
Full Name
TNF receptor-associated factor 1
Weight
46.164kDa
Alternative Names
EBI6; EBI6MGC:10353; Epstein-Bar virus-induced protein 6; Epstein-Barr virus-induced protein 6; TNF receptor-associated factor 1; TRAF1; TRAF-1 TRAF1 EBI6, MGC:10353 TNF receptor associated factor 1 TNF receptor-associated factor 1|Epstein-Bar virus-induced protein 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRAF1, check out the TRAF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRAF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRAF1 Antibody Picoband™ (A01365)
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No publications found for A01365
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