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Product Info Summary
| SKU: | A00424-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-TRAF3 Antibody
SKU/Catalog Number
A00424-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TRAF3 Antibody (Catalog # A00424-1). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TRAF3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TRAF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00424-1)
Host
Rabbit
Contents
TRAF3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TRAF3 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human. The immunogen is located within amino acids 20 - 70 of TRAF3.
Reactive Species
A00424-1 is reactive to TRAF3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
64.5 kDa
Background of TRAF3
Tumor necrosis factor (TNF) receptor associated factors (TRAFs) are the major signal transducers for the TNF receptor superfamily and the interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily. TRAF3 was first identified by its interaction with CD40 and the Epstein-Barr virus transforming protein LMP1. Several TRAF3 mRNA splice variants exist and some of these can activate the transcription factor NF-κB. Besides CD40, TRAF3 also interacts with the TRFR superfamily member lymphotoxin-beta receptor (LTbetaR) in association with TRAF2 and the apoptosis inhibitors cIAP1 and Smac. It has been suggested that TRAF3 induces mitochondria-mediated apoptosis upon binding of the TNF family cytokine LIGHT by LTbetaR.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00424-1 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TRAF3 antibody can be used for the detection of TRAF3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TRAF3 in HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 μg/mL.
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Immunocytochemistry of TRAF3 in HeLa cells with TRAF3 antibody at 10 μg/mL.
Specific Publications For Anti-TRAF3 Antibody (A00424-1)
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