Product Info Summary
SKU: | M04336 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, ICC, WB |
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Product info
Product Name
Anti-TRAF6BP Rabbit Monoclonal Antibody
SKU/Catalog Number
M04336
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-TRAF6BP Rabbit Monoclonal Antibody catalog # M04336. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAF6BP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04336)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
23T27
Isotype
IgG
Immunogen
A synthesized peptide derived from human TRAF6BP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04336 is reactive to TAX1BP1 in Human, Mouse, Rat
Applications
M04336 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
91 kDa
Calculated molecular weight
90.877kDa
Background of TAX1BP1
Plays a major role in tight junction-specific obliteration of the intercellular space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TRAF6BP using anti-TRAF6BP antibody (M04336).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human SIHA whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF6BP antigen affinity purified monoclonal antibody (Catalog # M04336) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF6BP at approximately 91 kDa. The expected band size for TRAF6BP is at 91 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TAX1BP1 (Source: Uniprot.org, NCBI)
Gene Name
TAX1BP1
Full Name
Tax1-binding protein 1
Weight
90.877kDa
Alternative Names
T6BP; Tax1 (human T-cell leukemia virus type I) binding protein 1; tax1-binding protein 1; TRAF6-binding protein; TXBP151CALCOCO3 TAX1BP1 CALCOCO3, T6BP, TXBP151 Tax1 binding protein 1 tax1-binding protein 1|TRAF6-binding protein|Tax1 (human T-cell leukemia virus type I) binding protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TAX1BP1, check out the TAX1BP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TAX1BP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRAF6BP Rabbit Monoclonal Antibody (M04336)
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No publications found for M04336
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Customer Reviews
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