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Product Info Summary
| SKU: | FEK0464 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin) and saliva |
| Assay Time: | 90 Minutes |
How Quick Picokine(R) ELISA kits work: Get data in 90 minutes
Product info
Product Name
Human MMP-8 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0464
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MMP-8 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests).Human MMP-8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human MMP-8 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0464)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: F21-G467
Capture/Detection Antibodies
The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0464 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0464 is reactive to MMP8 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin) and saliva
Application Guarantee
FEK0464 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP8
Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0464-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0464-ST | Human MMP8 Standard | 2 | 10 ng/tube |
| FEK0464-A | Human MMP8 Antibody A | 1 | 3ml |
| FEK0464-B | Human MMP8 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.025 | 0.082 | 0.133 | 0.229 | 0.412 | 0.681 | 1.208 | 1.96 |
Data Example Images
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Human MMP-8 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MMP-8 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 285 | 995 | 3376 | 197 | 1130 | 4869 |
| Standard deviation | 14.54 | 25.87 | 248.81 | 7.41 | 78.08 | 383.1 |
| CV (%) | 5.1% | 2.6% | 7.4% | 3.8% | 6.9% | 7.9% |
Protein Target Info & Infographic
Gene/Protein Information For MMP8 (Source: Uniprot.Org, NCBI)
Gene Name
MMP8
Full Name
Weight
53.4 kDa
For more info on MMP8, check out the MMP8 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us support@bosterbio.com.
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