Anti-Transcription factor E2F3 E2F3 Antibody

Product Name

Anti-Transcription factor E2F3 E2F3 Antibody

View all E2F3 Antibodies

SKU/Catalog Number

A03068-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Transcription factor E2F3 E2F3 Antibody (Catalog # A03068-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

E2F3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Transcription factor E2F3 E2F3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03068-1)

Host

Rabbit

Contents

E2F3 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E2F3 antibody was raised against a 15 amino acid peptide near the center of human E2F3. The immunogen is located within amino acids 150 - 200 of E2F3.

Cross-reactivity

E2F3 antibody is human and mouse reactive. At least three isoforms of E2F3 are known to exist; this antibody will detect only the largest isoform. This antibody is predicted to not cross-react with other members of the E2F transcription factor family.

Reactive Species

A03068-1 is reactive to E2F3 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

49.2 kDa

Background of E2F3

The E2F transcription factor 3 (E2F3) is a member of a small family of transcription factors that function through binding of DP interaction partner proteins. E2F3 recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle (1). Like the related E2F1 and E2F2, E2F3 is essential for cellular proliferation and progression through the cell cycle (2). Altered copy number and activity of this gene have been observed in a number of human cancers (3).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03068-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

E2F3 antibody can be used for detection of E2F3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information E2F3

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