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Product Info Summary
| SKU: | A13159 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Transmembrane protein 214 TMEM214 Antibody
SKU/Catalog Number
A13159
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Transmembrane protein 214 TMEM214 Antibody (Catalog # A13159). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TMEM214 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Transmembrane protein 214 TMEM214 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13159)
Host
Rabbit
Contents
TMEM214 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
TMEM214 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TMEM214. The immunogen is located within amino acids 70 - 120 of TMEM214.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of LRRTM4 are known to exist; this antibody will both isoforms. LRRTM4 antibody is predicted to not cross-react with other LRRTM family members.
Reactive Species
A13159 is reactive to TMEM214 in Human, Mouse, Rat
Applications
A13159 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
77.151kDa
Background of TMEM214
Transmembrane protein 214 (TMEM214) is a 77kD membrane protein which is widely expressed at high level. The gene encoding this protein maps to chromosome 2, at 2p23.3 TMEM214 interacts with LSM1, an SM-like protein, forming a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. The detailed function of TMEM214 is still unknown, but a recent study showed that TMEM214 may be used to explore the contribution of human host factors of some infectious diseases such as Dengue fever, West Nile fever and yellow fever. Multiple isoforms of TMEM214 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TMEM214 antibody can be used for detection of TMEM214 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TMEM214 in A20 cell lysate with TMEM214 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of TMEM214 in A20 cells with TMEM214 antibody at 5 μg/mL.
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Immunofluorescence of TMEM214 in A20 cells with TMEM214 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TMEM214 (Source: Uniprot.org, NCBI)
Gene Name
TMEM214
Full Name
Transmembrane protein 214
Weight
77.151kDa
Superfamily
TMEM214 family
Alternative Names
FLJ20254; FLJ39682; PP446; transmembrane protein 214 TMEM214 transmembrane protein 214 transmembrane protein 214
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TMEM214, check out the TMEM214 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TMEM214: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transmembrane protein 214 TMEM214 Antibody (A13159)
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