Product Info Summary
| SKU: | A13159 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Transmembrane protein 214 TMEM214 Antibody
SKU/Catalog Number
A13159
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Transmembrane protein 214 TMEM214 Antibody (Catalog # A13159). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TMEM214 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Transmembrane protein 214 TMEM214 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13159)
Host
Rabbit
Contents
TMEM214 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TMEM214 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TMEM214. The immunogen is located within amino acids 70 - 120 of TMEM214.
Reactive Species
A13159 is reactive to TMEM214 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
77.2 kDa
Background of TMEM214
Transmembrane protein 214 (TMEM214) is a 77kD membrane protein which is widely expressed at high level. The gene encoding this protein maps to chromosome 2, at 2p23.3 TMEM214 interacts with LSM1, an SM-like protein, forming a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. The detailed function of TMEM214 is still unknown, but a recent study showed that TMEM214 may be used to explore the contribution of human host factors of some infectious diseases such as Dengue fever, West Nile fever and yellow fever. Multiple isoforms of TMEM214 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13159 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TMEM214 antibody can be used for detection of TMEM214 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TMEM214 in A20 cell lysate with TMEM214 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of TMEM214 in A20 cells with TMEM214 antibody at 20 μg/mL.
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Immunocytochemistry of TMEM214 in A20 cells with TMEM214 antibody at 5 μg/mL.
Specific Publications For Anti-Transmembrane protein 214 TMEM214 Antibody (A13159)
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