Anti-TRAPB/SSR2 Antibody Picoband™

Product Name

Anti-TRAPB/SSR2 Antibody Picoband™

View all SSR2 Antibodies

SKU/Catalog Number

A11273-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRAPB/SSR2 Antibody Picoband™ catalog # A11273-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-TRAPB/SSR2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11273-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TRAPB/SSR2 recombinant protein (Position: E18-E138).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A11273-1 is reactive to SSR2 in Human, Mouse, Rat

Applications

A11273-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

22 kDa

Calculated molecular weight

20.135kDa

Background of SSR2

Translocon-associated protein subunit beta also known as TRAP-beta is a protein that in humans is encoded by the SSR2 gene. The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein (alpha-SSR or SSR1) and a 22-kD glycoprotein (beta-SSR or SSR2). The human beta-signal sequence receptor gene (SSR2) maps to chromosome bands 1q21-q23.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of TRAPB/SSR2 using anti-TRAPB/SSR2 antibody (A11273-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human COLO 320 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAPB/SSR2 antigen affinity purified polyclonal antibody (Catalog # A11273-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAPB/SSR2 at approximately 22 kDa. The expected band size for TRAPB/SSR2 is at 20 kDa.

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Figure 2. Flow Cytometry analysis of THP-1 cells using anti-TRAPB/SSR2 antibody (A11273-1).
Overlay histogram showing THP-1 cells stained with A11273-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAPB/SSR2 Antibody (A11273-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 3. Flow Cytometry analysis of U20S cells using anti-TRAPB/SSR2 antibody (A11273-1).
Overlay histogram showing U20S cells stained with A11273-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAPB/SSR2 Antibody (A11273-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SSR2 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp686F19123; Signal sequence receptor subunit beta; signal sequence receptor, beta (translocon-associated protein beta); SSR-beta; TLAP; translocon-associated protein beta; translocon-associated protein subunit beta; TRAP-beta; TRAPBTRAP-BETA SSR2 HSD25, TLAP, TRAP-BETA, TRAPB signal sequence receptor subunit 2 translocon-associated protein subunit beta|SSR-beta|signal sequence receptor subunit beta|signal sequence receptor, beta (translocon-associated protein beta)|translocon-associated protein beta

For more info on SSR2, check out the SSR2 Infographic

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In this infographic, you will see the following information for SSR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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