Anti-TREM1 Biotinylated Antibody

TREM1 antibody

Boster Bio Anti-TREM1 Biotinylated Antibody catalog # A02135-Biotin. Tested in ELISA applications. This antibody reacts with Human.

Product Info Summary

SKU: A02135-Biotin
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA

Product Name

Anti-TREM1 Biotinylated Antibody

View all TREM1 Antibodies

SKU/Catalog Number

A02135-Biotin

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TREM1 Biotinylated Antibody catalog # A02135-Biotin. Tested in ELISA applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TREM1 Biotinylated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02135-Biotin)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived huamn TREM1 recombinant protein (Position: A21-R200).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02135-Biotin is reactive to TREM1 in Human

Applications

A02135-Biotin is guaranteed for ELISA Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

26.387kDa

Background of TREM1

Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40). Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA Detection (Matched Antibody Pair), 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For TREM1 (Source: Uniprot.org, NCBI)

Gene Name

TREM1

Full Name

Triggering receptor expressed on myeloid cells 1

Weight

26.387kDa

Alternative Names

CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1 TREM1 CD354, TREM-1 triggering receptor expressed on myeloid cells 1 triggering receptor expressed on myeloid cells 1|triggering receptor expressed on monocytes 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TREM1, check out the TREM1 Infographic

TREM1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TREM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A02135-Biotin

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-TREM1 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

Be the first to review Anti-TREM1 Biotinylated Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Anti-TREM1 Biotinylated Antibody

Question

My question regarding product A02135-Biotin, anti-TREM1 Biotinylated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02135-Biotin anti-TREM1 Biotinylated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-18

Question

Does anti-TREM1 Biotinylated antibody A02135-Biotin work on zebrafish ELISA with blood?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

Our lab technicians have not validated anti-TREM1 Biotinylated antibody A02135-Biotin on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-TREM1 Biotinylated antibody A02135-Biotin to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish blood in ELISA, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-13

Question

I see that the anti-TREM1 Biotinylated antibody A02135-Biotin works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-12

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for monocyte neutrophil using anti-TREM1 Biotinylated antibody A02135-Biotin. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-02

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-02

Question

Will anti-TREM1 Biotinylated antibody A02135-Biotin work for ELISA with monocyte neutrophil?

M. Singh

Verified customer

Asked: 2018-09-21

Answer

According to the expression profile of monocyte neutrophil, TREM1 is highly expressed in monocyte neutrophil. So, it is likely that anti-TREM1 Biotinylated antibody A02135-Biotin will work for ELISA with monocyte neutrophil.

Boster Scientific Support

Answered: 2018-09-21

Question

I have attached the WB image, lot number and protocol we used for monocyte neutrophil using anti-TREM1 Biotinylated antibody A02135-Biotin. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-10-25

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-25

Question

Will A02135-Biotin anti-TREM1 Biotinylated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Anderson

Verified customer

Asked: 2014-02-13

Answer

As indicated on the product datasheet, A02135-Biotin anti-TREM1 Biotinylated antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-02-13

Order DetailsPrice
A02135-Biotin

100μg

$515
A02135-Biotin-carrier-free

Carrier Free

$515

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
A02135-Biotin
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$515.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.