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Product Info Summary
| SKU: | A02135-Biotin |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA |
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Product info
Product Name
Anti-TREM1 Biotinylated Antibody
SKU/Catalog Number
A02135-Biotin
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TREM1 Biotinylated Antibody catalog # A02135-Biotin. Tested in ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TREM1 Biotinylated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02135-Biotin)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived huamn TREM1 recombinant protein (Position: A21-R200).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02135-Biotin is reactive to TREM1 in Human
Applications
A02135-Biotin is guaranteed for ELISA Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
26.387kDa
Background of TREM1
Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40). Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA Detection (Matched Antibody Pair), 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For TREM1 (Source: Uniprot.org, NCBI)
Gene Name
TREM1
Full Name
Triggering receptor expressed on myeloid cells 1
Weight
26.387kDa
Alternative Names
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1 TREM1 CD354, TREM-1 triggering receptor expressed on myeloid cells 1 triggering receptor expressed on myeloid cells 1|triggering receptor expressed on monocytes 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TREM1, check out the TREM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TREM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TREM1 Biotinylated Antibody (A02135-Biotin)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-TREM1 Biotinylated Antibody
Question
My question regarding product A02135-Biotin, anti-TREM1 Biotinylated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02135-Biotin anti-TREM1 Biotinylated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-18
Question
Does anti-TREM1 Biotinylated antibody A02135-Biotin work on zebrafish ELISA with blood?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
Our lab technicians have not validated anti-TREM1 Biotinylated antibody A02135-Biotin on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-TREM1 Biotinylated antibody A02135-Biotin to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish blood in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-13
Question
I see that the anti-TREM1 Biotinylated antibody A02135-Biotin works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for monocyte neutrophil using anti-TREM1 Biotinylated antibody A02135-Biotin. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-02
Question
Will anti-TREM1 Biotinylated antibody A02135-Biotin work for ELISA with monocyte neutrophil?
M. Singh
Verified customer
Asked: 2018-09-21
Answer
According to the expression profile of monocyte neutrophil, TREM1 is highly expressed in monocyte neutrophil. So, it is likely that anti-TREM1 Biotinylated antibody A02135-Biotin will work for ELISA with monocyte neutrophil.
Boster Scientific Support
Answered: 2018-09-21
Question
I have attached the WB image, lot number and protocol we used for monocyte neutrophil using anti-TREM1 Biotinylated antibody A02135-Biotin. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-25
Question
Will A02135-Biotin anti-TREM1 Biotinylated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Anderson
Verified customer
Asked: 2014-02-13
Answer
As indicated on the product datasheet, A02135-Biotin anti-TREM1 Biotinylated antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-02-13
