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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)|
|Assay Time:||90 Minutes|
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Mouse TNF Alpha/Tumor necrosis factor PicoKine™ Quick ELISA Kit
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse TNF Alpha/Tumor necrosis factor PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse TNF Alpha/Tumor necrosis factor PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0527)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: L80-L235
15.6 pg/ml - 1,000 pg/ml (cell culture supernatants), 7.8 pg/ml - 500 pg/ml (mouse serum, plasma)
There is no detectable cross-reactivity with other relevant proteins.
FEK0527 is reactive to Tnf in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
FEK0527 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|FEK0527-CAP||96-well plate precoated with anti-Mouse TNF-alpha antibody||1|
|FEK0527-ST||lyophilized recombinant Mouse TNF-alpha standard||10 ng/tube|
|FEK0527-DA||Biotinylated anti-Mouse TNF-alpha detection antibody (50x)||130 ul|
|-||Avidin-Biotin-Peroxidase Complex (30x)||400 µl|
|AR1106-1||Sample diluent||30 ml|
|AR1106-2||Antibody diluent||12 ml|
|-||Avidin-Biotin-Peroxidase Diluent||12 ml|
|AR1106-5||Wash Buffer (25x)||20 ml|
|AR1104||Color Developing Reagent (TMB)||10 ml|
|AR1105||Stop Solution||10 ml|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of TNF-alpha in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Tnf (Source: Uniprot.Org, NCBI)
Tumor necrosis factor
tumor necrosis factor family
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha Tnf|DI, DIF, TNF-, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tna, Tnfs, Tnfsf1a, Tnlg1f, Tnf|tumor necrosis factor|tumor necrosis factor|cachectin|tumor necrosis factor ligand 1f|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Tnf, check out the Tnf Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tnf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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