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Product Info Summary
| SKU: | PB10105 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TREX2 Antibody Picoband®
SKU/Catalog Number
PB10105
PB1158 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TREX2 Antibody Picoband® catalog # PB10105. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TREX2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10105)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TREX2 recombinant protein (Position: E69-A279). Human TREX2 shares 88.6% amino acid (aa) sequence identity with mouse TREX2.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10105 is reactive to TREX2 in Human
Observed Molecular Weight
37 kDa
Calculated molecular weight
25.9 kDa
Background of TREX2
Three prime repair exonuclease 2 is an enzyme that in humans is encoded by the TREX2 gene. By genomic sequence analysis, the TREX2 gene is mapped to chromosome Xq28. This gene encodes a protein with 3' exonuclease activity. Enzymes with this activity are involved in DNA replication, repair, and recombination. Similarity to an E. coli protein suggests that this enzyme may be a subunit of DNA polymerase III, which does not have intrinsic exonuclease activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10105 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA whole cell, MCF-7 whole cell
Validation Images & Assay Conditions
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Western blot analysis of TREX2 using anti-TREX2 antibody (PB10105).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: MCF-7 whole cell lysates lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREX2 antigen affinity purified polyclonal antibody (Catalog # PB10105) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREX2 at approximately 37 kDa. The expected band size for TREX2 is at 31 kDa.
Specific Publications For Anti-TREX2 Antibody Picoband® (PB10105)
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Customer Q&As
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4 Customer Q&As for Anti-TREX2 Antibody Picoband®
Question
Would PB10105 anti-TREX2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
As indicated on the product datasheet, PB10105 anti-TREX2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-27
Question
We are currently using anti-TREX2 antibody PB10105 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
The anti-TREX2 antibody (PB10105) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-11
Question
Is there a BSA free version of anti-TREX2 antibody PB10105 available?
M. Rodriguez
Verified customer
Asked: 2015-07-22
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TREX2 antibody PB10105 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-07-22
Question
I am interested in to test anti-TREX2 antibody PB10105 on human skin of leg for research purposes, then I may be interested in using anti-TREX2 antibody PB10105 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Carter
Verified customer
Asked: 2014-12-04
Answer
The products we sell, including anti-TREX2 antibody PB10105, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-12-04
