Anti-TRI18 MID1 Antibody

Boster Bio Anti-TRI18 MID1 Antibody catalog # A01774. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01774
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, WB

Product Name

Anti-TRI18 MID1 Antibody

See all MID1 products

SKU/Catalog Number

A01774

Size

100μl

Form

Liquid

Description

Boster Bio Anti-TRI18 MID1 Antibody catalog # A01774. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRI18 MID1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01774)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from internal of human TRI18.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01774 is reactive to MID1 in Human, Mouse, Rat

Applications

A01774 is guaranteed for IF, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:3000
IHC, 1:50-1:100
Immunofluorescence, 1:100-1:500

Validation Images & Assay Conditions

Gene/Protein Information For MID1 (Source: Uniprot.Org, NCBI)

Uniprot ID

O15344

Gene ID

4281

Gene Name

MID1

Full Name

E3 ubiquitin-protein ligase Midline-1

Weight

75.251kDa

Superfamily

TRIM/RBCC family

Alternative Names

EC 6.3.2.-; FLJ57031; FLJ58683; FLJ76288; FXYGBBB1; Midin; midline 1 (Opitz/BBB syndrome); RNF59MIDIN; TRIM18mouse, homolog of MID1 BBBG1, FXY, GBBB1, MIDIN, OGS1, OS, OSX, RNF59, TRIM18, XPRF, ZNFXY midline 1 E3 ubiquitin-protein ligase Midline-1|Opitz/BBB syndrome|RING finger protein 59|RING finger protein Midline-1|RING-type E3 ubiquitin transferase Midline-1|midline 1 RING finger protein|putative transcription factor XPRF|tripartite motif protein TRIM18|tripartite motif-containing protein 18|zinc finger on X and Y, mouse, homolog of

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MID1, check out the MID1 Infographic

MID1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MID1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Anti-TRI18 MID1 Antibody

Question

Is a blocking peptide available for product anti-TRI18 antibody (A01774)?

W. Parker

Verified customer

Asked: 2020-03-11

Answer

We do provide the blocking peptide for product anti-TRI18 antibody (A01774). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-11

Question

Will A01774 anti-TRI18 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Miller

Verified customer

Asked: 2020-01-14

Answer

You can see on the product datasheet, A01774 anti-TRI18 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-14

Question

you antibody to test anti-TRI18 antibody A01774 on rat fetal kidney for research purposes, then I may be interested in using anti-TRI18 antibody A01774 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

The products we sell, including anti-TRI18 antibody A01774, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-13

Question

Will anti-TRI18 antibody A01774 work for IHC with fetal kidney?

Verified Customer

Verified customer

Asked: 2017-05-22

Answer

According to the expression profile of fetal kidney, MID1 is highly expressed in fetal kidney. So, it is likely that anti-TRI18 antibody A01774 will work for IHC with fetal kidney.

Boster Scientific Support

Answered: 2017-05-22

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A01774
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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