Anti-TRI18 MID1 Antibody

Product Name

Anti-TRI18 MID1 Antibody

View all MID1 Antibodies

SKU/Catalog Number

A01774

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-TRI18 MID1 Antibody (Catalog # A01774). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRI18 MID1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01774)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human TRI18.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01774 is reactive to MID1 in Human, Mouse, Rat

Applications

A01774 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

75.251kDa

Background of MID1

Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.

Quaderi N.A., Nat. Genet. 17:285-291(1997).
Perry J., Hum. Mol. Genet. 7:299-305(1998).
Van den Veyver I.B., Genomics 51:251-261(1998).<

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

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Immunohistochemistry analysis of paraffin-embedded human brain tissue using TRI18 antibody A01774.

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Western blot analysis of extracts from 293 cells, using TRI18 antibody A01774.

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Immunofluorescence analysis of HeLa cells, using TRI18 antibody A01774.

Gene/Protein Information For MID1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 6.3.2.-; FLJ57031; FLJ58683; FLJ76288; FXYGBBB1; Midin; midline 1 (Opitz/BBB syndrome); RNF59MIDIN; TRIM18mouse, homolog of MID1 BBBG1, FXY, GBBB1, MIDIN, OGS1, OS, OSX, RNF59, TRIM18, XPRF, ZNFXY midline 1 E3 ubiquitin-protein ligase Midline-1|Opitz/BBB syndrome|RING finger protein 59|RING finger protein Midline-1|RING-type E3 ubiquitin transferase Midline-1|midline 1 RING finger protein|putative transcription factor XPRF|tripartite motif protein TRIM18|tripartite motif-containing protein 18|zinc finger on X and Y, mouse, homolog of

For more info on MID1, check out the MID1 Infographic

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In this infographic, you will see the following information for MID1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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