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Product Info Summary
| SKU: | M11223 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-TRIM Purified TRAT1 Monoclonal Antibody
SKU/Catalog Number
M11223
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TRIM Purified TRAT1 Monoclonal Antibody (Catalog# M11223). Tested in Flow Cytometry, IP, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-TRIM Purified TRAT1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11223)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
TRIM-04
Isotype
Mouse IgG2a
Immunogen
Recombinant intracellular domain (aa 29-186) of human TRIM. The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M11223 is reactive to TRAT1 in Human
Applications
M11223 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
21.211kDa
Background of TRIM
TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 µg/ml, intracellular staining.
Western blotting: Non-reducing conditions.
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-TRIM Purified TRAT1 Monoclonal Antibody M11223
Western blotting analysis of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sampleProtein Target Info & Infographic
Gene/Protein Information For TRAT1 (Source: Uniprot.org, NCBI)
Gene Name
TRAT1
Full Name
T-cell receptor-associated transmembrane adapter 1
Weight
21.211kDa
Alternative Names
pp29/30; T cell receptor associated transmembrane adaptor 1; T cell receptor interacting molecule; T-cell receptor interacting molecule; T-cell receptor-associated transmembrane adapter 1; T-cell receptor-interacting molecule; TCRIM; TRAT1; TRIM; TRIMHSPC062 TRAT1 HSPC062, TCRIM, TRIM, pp29/30 T cell receptor associated transmembrane adaptor 1 T-cell receptor-associated transmembrane adapter 1|T cell receptor interacting molecule
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRAT1, check out the TRAT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRIM Purified TRAT1 Monoclonal Antibody (M11223)
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No publications found for M11223
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