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Product Info Summary
| SKU: | A02079 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-TRIM21 Antibody
SKU/Catalog Number
A02079
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TRIM21 Antibody (Catalog # A02079). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
TRIM21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-TRIM21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02079)
Host
Rabbit
Contents
TRIM21 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-TRIM21 antibody was raised against a peptide corresponding to 16 amino acids near the center of human TRIM21. The immunogen is located within amino acids 290-340 of TRIM21.
Cross-reactivity
TRIM21 antibody is human specific. This antibody is predicted to not cross-react with TRIM6.
Reactive Species
A02079 is reactive to TRIM21 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
54.2 kDa
Background of TRIM21
TRIM21, a member of the tripartite motif (TRIM) family, is a part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus (1). RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus (2). TRIM21 also acts as a cytosolic antibody receptor; detection of intracellular antibodies activate immune signaling by stimulating the transcription factor pathways of NF-kappaB, AP-1, IRF3, IRF5, and IRF7, resulting in the production of proinflammatory cytokines and the induction of an antiviral state (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02079 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5-2 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Human Lung Tissue Lysate in (Lane 1) the absence and (Lane 2) the presence of blocking peptide
Loading: 15 μg of lysates per lane.
Antibodies: TRIM21, A02079 (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: TRIM21, A02079 (0.5 μg/mL), TRIM21, 18-413 (2 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of TRIM21 in Human Small Intestine Tissue
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-TRIM21 antibody (A02079) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of TRIM21 in Human Small Intestine Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human small intestine tissue labeling TRIM21 with A02079 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Specific Publications For Anti-TRIM21 Antibody (A02079)
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