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TRIM24 antibody (M03258-2) performs well in IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of TRIM24 using anti-TRIM24 antibody (M03258-2).
TRIM24 was detected in a paraffin-embedded section of human adenocarcinoma of the colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-TRIM24 Antibody (M03258-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
TRIM24 was detected in a paraffin-embedded section of human adenocarcinoma of the colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-TRIM24 Antibody (M03258-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Designing a Western blot or IHC for TRIM24? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2)
TRIM24 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | IF, IHC, ICC, WB |
Tested Applications
TRIM24 antibody was positively detected by WB in:
human Hela whole cell, human MCF-7 whole cell, human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TRIM24 antibody was positively detected by IHC in:
human adenocarcinoma of the colon tissue, human thyroid cancer tissue, human ovarian serous tumor tissue, human breast cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TRIM24 antibody was positively detected by ICC/IF in:
MCF-7 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2)
View all TRIM24 Antibodies
View all TRIM24 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
4G6C2
Isotype
Mouse IgG1
Immunogen
E.coli-derived human TRIM24 recombinant protein (Position: A706-D964).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
130 kDa
Calculated Molecular Weight
116.8 kDa
Background of TRIM24
Tripartite motif-containing 24 (TRIM24) also known as transcriptional intermediary factor 1α (TIF1α) is a protein that, in humans, is encoded by the TRIM24 gene. The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Cite This Product
Anti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03258-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Sheep
96%
High Homology
Dog
96%
High Homology
Mouse
94%
High Homology
Cow
94%
High Homology
Rat
93%
High Homology
Rabbit
92%
High Homology
Zebrafish
41%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TRIM24 antibody
Immunohistochemistry (IHC) protocol for TRIM24 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for TRIM24 antibody
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Product Promise
We promise Anti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2) performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M03258-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 16, 2026