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- Table of Contents
TRIP12 antibody (A05449) performs well in ELISA, IF, IHC-P, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of TRIP12 in rat colon tissue with TRIP12 antibody at 20 μg/ml.
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Anti-TRIP12 Antibody
TRIP12 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, ICC, WB |
Recommend Dilution
Application
Dilution
Species
TRIP12 antibody can be used for detection of TRIP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunocytochemistry start at 2 μg/mL.For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples; Immunocytochemistry in human samples; and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-TRIP12 Antibody
View all TRIP12 Antibodies
View all TRIP12 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Antibody can be stored at 4°C, stable for one year. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
TRIP12 antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TRIP12 antibody was raised against a 18 amino acid peptide near the carboxy terminus of human TRIP12. The immunogen is located within amino acids 1930 - 1980 of TRIP12.
Cross-Reactivity
TRIP12 antibody is human, mouse and rat reactive. At least four isoforms are known to exist.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
220.4 kDa
Background of TRIP12
Thyroid hormone receptors (TRs) are transcription factors that regulate the expression of specific genes in a hormone-dependent manner (1). TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent E3 ubiquitin ligase involved in the human ubiquitin fusion degradation (UFD) pathway and also modulates the NEDD8 pathway (2,3). TRIP12 contains one WWE domain and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain suggested to contain a noncovalent ubiquitin-binding site (4). TRIP12 acts as a key regulator of DNA damage response and the ubiquitin ligase activity of TRIP12 is essential for mouse development (5).
Cite This Product
Anti-TRIP12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05449)
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Product Promise
We promise Anti-TRIP12 Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A05449
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026