Anti-TRIP12 Antibody

Product Name

Anti-TRIP12 Antibody

View all TRIP12 Antibodies

SKU/Catalog Number

A05449

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TRIP12 Antibody (Catalog # A05449). Tested in ELISA, WB, IHC-P, IF, ICC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TRIP12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05449)

Host

Rabbit

Contents

TRIP12 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TRIP12 antibody was raised against a 18 amino acid peptide near the carboxy terminus of human TRIP12. The immunogen is located within amino acids 1930 - 1980 of TRIP12.

Cross-reactivity

TRIP12 antibody is human, mouse and rat reactive. At least four isoforms are known to exist.

Reactive Species

A05449 is reactive to TRIP12 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

220.4 kDa

Background of TRIP12

Thyroid hormone receptors (TRs) are transcription factors that regulate the expression of specific genes in a hormone-dependent manner (1). TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent E3 ubiquitin ligase involved in the human ubiquitin fusion degradation (UFD) pathway and also modulates the NEDD8 pathway (2,3). TRIP12 contains one WWE domain and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain suggested to contain a noncovalent ubiquitin-binding site (4). TRIP12 acts as a key regulator of DNA damage response and the ubiquitin ligase activity of TRIP12 is essential for mouse development (5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05449 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TRIP12 antibody can be used for detection of TRIP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunocytochemistry start at 2 μg/mL.For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples; Immunocytochemistry in human samples; and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TRIP12

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