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Product Info Summary
| SKU: | A01472-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-TRPC3 Antibody
SKU/Catalog Number
A01472-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TRPC3 Antibody (Catalog # A01472-1). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TRPC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01472-1)
Host
Rabbit
Contents
TRPC3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TRPC3 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human TRPC3. The immunogen is located within amino acids 120 - 170 of TRPC3.
Cross-reactivity
This antibody may cross-react with TRPC6 and TRPC7.
Reactive Species
A01472-1 is reactive to TRPC3 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
96.0 kDa
Background of TRPC3
The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC3/6/7 subfamily can be activated by diacylglycerol analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. TRPC3 encodes a Ca2+-permeant channel that is agonist-activated but not store-operated or directly receptor-activated. TRPC3 physically interacts with TRPC6 and TRPC7 and forms functional tetrameric channels.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01472-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TRPC3 in human heart tissue lysate with TRPC3 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of TRPC3 in mouse heart tissue with TRPC3 antibody at 10 μg/mL.
Specific Publications For Anti-TRPC3 Antibody (A01472-1)
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