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Product Info Summary
| SKU: | A03874-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TRPV3 Antibody Picoband®
SKU/Catalog Number
A03874-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRPV3 Antibody Picoband® catalog # A03874-2. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TRPV3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03874-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TRPV3, which shares 77.8% amino acid (aa) sequence identity with mouse TRPV3.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03874-2 is reactive to TRPV3 in Human
Observed Molecular Weight
100 kDa
Calculated molecular weight
90.6 kDa
Background of TRPV3
Transient receptor potential cation channel, subfamily V, member 3, also known as TRPV3, is a human gene encoding the protein of the same name. This gene product belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. This gene lies in close proximity to another family member gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03874-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Positive Control
WB: human MDA-MB-453 whole cell, human PC-3 whole cell, human Caco-2 whole cell, human Hela whole cell
Validation Images & Assay Conditions
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Western blot analysis of TRPV3 using anti-TRPV3 antibody (A03874-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MDA-MB-453 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV3 antigen affinity purified polyclonal antibody (Catalog # A03874-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV3 at approximately 100 kDa. The expected band size for TRPV3 is at 156 kDa.
Specific Publications For Anti-TRPV3 Antibody Picoband® (A03874-2)
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