Anti-Trypsin-1 PRSS1 Antibody

Trypsin 1/PRSS1 antibody

Boster Bio Anti-Trypsin-1 PRSS1 Antibody catalog # A01637. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A01637
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Trypsin-1 PRSS1 Antibody

View all Trypsin 1/PRSS1 Antibodies

SKU/Catalog Number

A01637

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Trypsin-1 PRSS1 Antibody catalog # A01637. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Trypsin-1 PRSS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01637)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

2C5

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human Trypsin-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01637 is reactive to PRSS1 in Human

Applications

A01637 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

26.558kDa

Background of Trypsin 1/PRSS1

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Fulton, D. et al. (1999) Nature 399, 597-601.
Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For PRSS1 (Source: Uniprot.org, NCBI)

Gene Name

PRSS1

Full Name

Trypsin-1

Weight

26.558kDa

Superfamily

peptidase S1 family

Alternative Names

Beta-trypsin; Cationic trypsinogen; digestive zymogen; DKFZp779A0837; EC 3.4.21; EC 3.4.21.4; FLJ57296; MGC120175; MGC149362; nonfunctional trypsin 1; protease, serine, 1 (trypsin 1); PRSS1; PTRYI; Serine protease 1; TRP1; TRY1beta-trypsin; TRY4; Trygn16; TRYP1; Trypsin 1; Trypsin I; trypsin-1; Trypsinogen 1; trypsinogen A PRSS1 TRP1, TRY1, TRY4, TRYP1 serine protease 1 trypsin-1|TCR V beta 4.1|beta-trypsin|cationic trypsinogen|digestive zymogen|nonfunctional trypsin 1|protease, serine 1|trypsinogen 1|trypsinogen A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRSS1, check out the PRSS1 Infographic

PRSS1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRSS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Trypsin-1 PRSS1 Antibody

Question

Does anti-Trypsin-1 antibody A01637 work on dog WB with gastric adenocarcinoma?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

Our lab technicians have not validated anti-Trypsin-1 antibody A01637 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Trypsin-1 antibody A01637 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog gastric adenocarcinoma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-08

Question

We are currently using anti-Trypsin-1 antibody A01637 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?

N. Edwards

Verified customer

Asked: 2019-08-06

Answer

The anti-Trypsin-1 antibody (A01637) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-06

Question

I am interested in to test anti-Trypsin-1 antibody A01637 on human prostate for research purposes, then I may be interested in using anti-Trypsin-1 antibody A01637 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-07-11

Answer

The products we sell, including anti-Trypsin-1 antibody A01637, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-07-11

Question

See below the WB image, lot number and protocol we used for prostate using anti-Trypsin-1 antibody A01637. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-04-06

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-06

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for prostate using anti-Trypsin-1 antibody A01637. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-07-07

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-07

Question

Is there a BSA free version of anti-Trypsin-1 antibody A01637 available?

T. Carter

Verified customer

Asked: 2014-12-17

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Trypsin-1 antibody A01637 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-12-17

Question

I see that the anti-Trypsin-1 antibody A01637 works with WB, what is the protocol used to produce the result images on the product page?

R. Li

Verified customer

Asked: 2014-06-23

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-06-23

Order DetailsPrice
A01637

100uL

$399

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A01637
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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