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Product Info Summary
| SKU: | M01233-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-TSG101 Antibody
SKU/Catalog Number
M01233-1
Size
50 µl
Description
Boster Bio Anti-TSG101 Antibody (Catalog # M01233-1). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TSG101 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01233-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1883CT788.47.15.46
Isotype
IgG1,κ
Immunogen
This antibody is generated from a mouse immunized with a recombinant protein from human.
Reactive Species
M01233-1 is reactive to TSG101 in Human
Calculated molecular weight
43.9 kDa
Background of TSG101
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01233-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-TSG101 at 1:2000 dilution
Lane 1: Human brain lysate
Lane 2: K562 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 44 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-TSG101 Antibody (M01233-1)
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