Click image to see more details
Product Info Summary
| SKU: | A30469 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TSG6 Antibody
SKU/Catalog Number
A30469
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TSG6 Antibody (Catalog# A30469). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TSG6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30469)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TSG6. Immunogen sequence location: 21-70
Reactive Species
A30469 is reactive to TNFAIP6 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
31.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30469 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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Western blot analysis of lysates from Jurkat, HeLa, and HepG2 cells, using TSG6 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TSG6 Antibody (A30469)
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Customer Q&As
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5 Customer Q&As for Anti-TSG6 Antibody
Question
Is a blocking peptide available for product anti-TSG6 antibody (A30469)?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
We do provide the blocking peptide for product anti-TSG6 antibody (A30469). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-10
Question
I was wanting to use to test anti-TSG6 antibody A30469 on human layer of synovial tissue for research purposes, then I may be interested in using anti-TSG6 antibody A30469 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
The products we sell, including anti-TSG6 antibody A30469, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-06
Question
Do you have a BSA free version of anti-TSG6 antibody A30469 available?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TSG6 antibody A30469 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-01
Question
I have a question about product A30469, anti-TSG6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30469 anti-TSG6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-26
Question
Will A30469 anti-TSG6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
You can see on the product datasheet, A30469 anti-TSG6 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-15
