Click image to see more details
Product Info Summary
| SKU: | M00290-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TTR/Prealbumin Rabbit Monoclonal Antibody
View all Transthyretin/Prealbumin Antibodies
SKU/Catalog Number
M00290-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TTR/Prealbumin Rabbit Monoclonal Antibody catalog # M00290-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TTR/Prealbumin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00290-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABOD-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TTR
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00290-1 is reactive to TTR in Human
Applications
M00290-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
59 kDa
Calculated molecular weight
15.887kDa
Background of Transthyretin/Prealbumin
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of TTR expression in human serum lysate.
Protein Target Info & Infographic
Gene/Protein Information For TTR (Source: Uniprot.org, NCBI)
Gene Name
TTR
Full Name
Transthyretin
Weight
15.887kDa
Superfamily
transthyretin family
Alternative Names
ATTR; HsT2651; PALB; PALBTBPA; Prealbumin; prealbumin, amyloidosis type I; TBPA; thyroxine-binding prealbumin; Transthyretin; TTR TTR ATTR, CTS, CTS1, HEL111, HsT2651, PALB, TBPA, TTN transthyretin transthyretin|epididymis luminal protein 111|prealbumin, amyloidosis type I|thyroxine-binding prealbumin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TTR, check out the TTR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TTR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TTR/Prealbumin Rabbit Monoclonal Antibody (M00290-1)
Hello CJ!
M00290-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of renal cell markers and detection of 3p loss links endolymphatic sac tumor to renal cell carcinoma and warrants careful evaluation to avoid %u2026
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TTR/Prealbumin Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-TTR/Prealbumin Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
17 Customer Q&As for Anti-TTR/Prealbumin Rabbit Monoclonal Antibody
Question
We ordered your anti-TTR/Prealbumin Rabbit Monoclonal antibody for WB on brain in the past. I am using human, and We intend to use the antibody for IP next. We are interested in examining brain as well as plasma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
I took a look at the website and datasheets of our anti-TTR/Prealbumin Rabbit Monoclonal antibody and I see that M00290-1 has been validated on human in both WB and IP. Thus M00290-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-05-07
Question
I see that the anti-TTR/Prealbumin Rabbit Monoclonal antibody M00290-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-27
Question
Is there a BSA free version of anti-TTR/Prealbumin Rabbit Monoclonal antibody M00290-1 available?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-TTR/Prealbumin Rabbit Monoclonal antibody M00290-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-21
Question
Does anti-TTR/Prealbumin Rabbit Monoclonal antibody M00290-1 work on dog WB with plasma?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
Our lab technicians have not validated anti-TTR/Prealbumin Rabbit Monoclonal antibody M00290-1 on dog. You can run a BLAST between dog and the immunogen sequence of anti-TTR/Prealbumin Rabbit Monoclonal antibody M00290-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog plasma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-27
Question
What is the epitope for M00290-1?
Verified customer
Asked: 2019-11-27
Answer
The immunogen sequence for the Anti-TTR/Prealbumin Rabbit Monoclonal Antibody (M00290-1) is CYSTTAVVTNPKE.
Boster Scientific Support
Answered: 2019-12-03
Question
Is this M00290-1 anti-TTR/Prealbumin Rabbit Monoclonal antibody reactive to the isotypes of TTR?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The immunogen of M00290-1 anti-TTR/Prealbumin Rabbit Monoclonal antibody is A synthesized peptide derived from human TTR. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-22
Question
Would anti-TTR/Prealbumin Rabbit Monoclonal antibody M00290-1 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
According to the expression profile of brain, TTR is highly expressed in brain. So, it is likely that anti-TTR/Prealbumin Rabbit Monoclonal antibody M00290-1 will work for WB with brain.
Boster Scientific Support
Answered: 2019-10-11
Question
We have observed staining in human plasma. Do you have any suggestions? Is anti-TTR/Prealbumin Rabbit Monoclonal antibody supposed to stain plasma positively?
P. Wu
Verified customer
Asked: 2019-01-09
Answer
From what I have seen in literature plasma does express TTR. From what I have seen in Uniprot.org, TTR is expressed in pigmented layer of retina, liver, retina, corpus callosum, platelet, brain, cajal-retzius cell fetal brain cortex, plasma, cerebrospinal fluid, among other tissues. Regarding which tissues have TTR expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1979335
Cerebrospinal fluid, Pubmed ID: 18221012
Corpus callosum, Pubmed ID: 14702039
Liver, Pubmed ID: 1666289, 2015890, 3022108, 6093805, 15489334, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Platelet, Pubmed ID: 12665801
Retina, Pubmed ID: 10328977
Boster Scientific Support
Answered: 2019-01-09
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-TTR/Prealbumin Rabbit Monoclonal antibody M00290-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-03-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-15
Question
We are currently using anti-TTR/Prealbumin Rabbit Monoclonal antibody M00290-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
The anti-TTR/Prealbumin Rabbit Monoclonal antibody (M00290-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-26
Question
I was wanting to use your anti-TTR/Prealbumin Rabbit Monoclonal antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
You can see on the product datasheet, M00290-1 anti-TTR/Prealbumin Rabbit Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-09
Question
Here is the WB image, lot number and protocol we used for brain using anti-TTR/Prealbumin Rabbit Monoclonal antibody M00290-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-05-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-30
Question
Can you help my question with product M00290-1, anti-TTR/Prealbumin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Parker
Verified customer
Asked: 2017-02-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00290-1 anti-TTR/Prealbumin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-02-21
Question
My colleagues were satisfied with the WB result of your anti-TTR/Prealbumin Rabbit Monoclonal antibody. However we have seen positive staining in pigmented layer of retina secreted. cytoplasm. using this antibody. Is that expected? Could you tell me where is TTR supposed to be expressed?
S. Krishna
Verified customer
Asked: 2015-11-16
Answer
From what I have seen in literature, pigmented layer of retina does express TTR. Generally TTR expresses in secreted. cytoplasm. Regarding which tissues have TTR expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1979335
Cerebrospinal fluid, Pubmed ID: 18221012
Corpus callosum, Pubmed ID: 14702039
Liver, Pubmed ID: 1666289, 2015890, 3022108, 6093805, 15489334, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Platelet, Pubmed ID: 12665801
Retina, Pubmed ID: 10328977
Boster Scientific Support
Answered: 2015-11-16
Question
My question regards to test anti-TTR/Prealbumin Rabbit Monoclonal antibody M00290-1 on human brain for research purposes, then I may be interested in using anti-TTR/Prealbumin Rabbit Monoclonal antibody M00290-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
O. Walker
Verified customer
Asked: 2014-05-20
Answer
The products we sell, including anti-TTR/Prealbumin Rabbit Monoclonal antibody M00290-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-05-20
Question
Is a blocking peptide available for product anti-TTR/Prealbumin Rabbit Monoclonal antibody (M00290-1)?
J. Wu
Verified customer
Asked: 2013-05-31
Answer
We do provide the blocking peptide for product anti-TTR/Prealbumin Rabbit Monoclonal antibody (M00290-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-05-31
Question
Will M00290-1 anti-TTR/Prealbumin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Johnson
Verified customer
Asked: 2013-01-21
Answer
You can see on the product datasheet, M00290-1 anti-TTR/Prealbumin Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-01-21
