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Product Info Summary
| SKU: | A10831 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TUBB6 Antibody (Center)
SKU/Catalog Number
A10831
Size
80 µl
Description
Boster Bio Anti-TUBB6 Antibody (Center) (Catalog # A10831). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TUBB6 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10831)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TUBB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139-166 amino acids from the Central region of human TUBB6.
Reactive Species
A10831 is reactive to TUBB6 in Human
Calculated molecular weight
49.9 kDa
Background of TUBB6
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10831 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-TUBB6 Antibody (Center) at 1:2000 dilution
Lane 1: K562 whole cell lysate
Lane 2: MDA-MB-231 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: A431 whole cell lysate
Lane 5: A549 whole cell lysate
Lane 6: U-251MG whole cell lysate
Lane 7: MCF-7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-TUBB6 Antibody (Center) (A10831)
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