Product Info Summary
SKU: | A03989-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Tubulin alpha Antibody Picoband®
View all alpha Tubulin Antibodies
SKU/Catalog Number
A03989-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tubulin alpha Antibody Picoband® catalog # A03989-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tubulin alpha Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03989-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Tubulin alpha recombinant protein (Position: N18-A403).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03989-1 is reactive to TUBA1A/TUBA1B/TUBA1C in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
50.136kDa
Background of alpha Tubulin
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03989-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human HEK293 whole cell, human HepG2 whole cell, human SW620 whole cell, human Raji whole cell, human K562 whole cell, rat brain tissue, mouse brain tissue
IHC: human placenta tissue
ICC/IF: A431 cell, NRK cell
FCM: Hela cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Tubulin alpha using anti-Tubulin alpha antibody (A03989-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human SW620 whole cell lysates,
Lane 6: human Raji whole cell lysates,
Lane 7: human K562 whole cell lysates,
Lane 8: rat brain tissue lysates,
Lane 9: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tubulin alpha antigen affinity purified polyclonal antibody (Catalog # A03989-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tubulin alpha at approximately 55KD. The expected band size for Tubulin alpha is at 55KD.
Click image to see more details
Figure 2. IHC analysis of Tubulin alpha using anti Tubulin alpha antibody (A03989-1).
Tubulin alpha was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Tubulin alpha Antibody (A03989-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IF analysis of Tubulin alpha using anti-Tubulin alpha antibody (A03989-1).
Tubulin alpha was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Tubulin alpha Antibody (A03989-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 4. IF analysis of Tubulin alpha using anti-Tubulin alpha antibody (A03989-1).
Tubulin alpha was detected in immunocytochemical section of NRK cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Tubulin alpha Antibody (A03989-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Flow Cytometry analysis of Hela cells using anti-Tubulin alpha antibody (A03989-1).
Overlay histogram showing Hela cells stained with A03989-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tubulin alpha Antibody (A03989-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TUBA1A/TUBA1B/TUBA1C (Source: Uniprot.org, NCBI)
Gene Name
TUBA1A/TUBA1B/TUBA1C
Full Name
Tubulin alpha-1A chain
Weight
50.136kDa
Superfamily
tubulin family
Alternative Names
Intercellular adhesion molecule 2; ICAM-2; CD102; ICAM2 TUBA1A B-ALPHA-1, LIS3, TUBA3 tubulin alpha 1a tubulin alpha-1A chain|hum-a-tub1|hum-a-tub2|tubulin B-alpha-1|tubulin alpha-3 chain|tubulin, alpha, brain-specific
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBA1A/TUBA1B/TUBA1C, check out the TUBA1A/TUBA1B/TUBA1C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBA1A/TUBA1B/TUBA1C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tubulin alpha Antibody Picoband® (A03989-1)
Hello CJ!
A03989-1 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Construction of Biofunctionalized Anisotropic Hydrogel Micropatterns and Their Effect on Schwann Cell Behavior in Peripheral Nerve Regeneration
Yu-Yuan Chen,Yin-Peng Bai,Bin Li,Xiao-Bo Zhao,Cheng-Jie Yang,Ying-Qian Liu,Jian-Mei Gao,Jun Guo,Chun Li,Jing-Wen Peng,Zhong-Min Zhao,Zhi-Jun Zhang,Chuan-Rui Xu,Design and Synthesis of Novel 20(S)-α-aminophosphonate Derivatives of Camptothecin as Potent Antitumor Agents,Bioorganic Chemistry,2021,105065,ISSN 0045-2068, https://doi.org/10.1016/j.bioorg.2021.105065.
Species: Human,Mouse
A03989-1 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:1:1000
Zhu J,Lv Y,Hao J,Shi T,Wang S,Wang K,Fan X,Guo Y,Zhang J,Li J.N-myc downstream-regulated gene 2 promotes the protein stability of estrogen receptor beta via inhibition of ubiquitin-protein ligase E3A to suppress colorectal cancer.J Gastrointest Oncol.2020
Species: Human,Mouse
A03989-1 usage in article: APP:WB, SAMPLE:COLON TISSUE,HCT116 CELL AND SW620 CELL, DILUTION:1:1000
Yang Z,Zhao X,Shang W,Liu Y,Ji JF,Liu JP,Tong C.Pyrroline-5-carboxylate synthase senses cellular stress and modulates metabolism by regulating mitochondrial respiration.Cell Death Differ.2020 Aug 7.doi:10.1038/s41418-020-0601-5.Epub ahead of print.PMID:32
Species: Drosophila,Human
A03989-1 usage in article: APP:WB, SAMPLE:IMR-90 CELL, DILUTION:NA
Ni S,Kuang Y,Yuan Y,Yu B. Mitochondrion-mediated iron accumulation promotes carcinogenesis and Warburg effect through reactive oxygen species in osteosarcoma. Cancer Cell Int.2020 Aug 18;20:399. doi:10.1186/s12935-020-01494-3.PMID:32831652;PMCID:PMC743701
Species: Human,Mouse
A03989-1 usage in article: APP:WB, SAMPLE:SAOS-2 CELL AND U2OS CELL, DILUTION:1:1000
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